Macrophage immigration inhibitory factor promotes cell proliferation and inhibits apoptosis of cervical adenocarcinoma
Macrophage immigration inhibitory factor promotes cell proliferation and inhibits apoptosis of cervical adenocarcinoma
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巨噬细胞迁移抑制因子促进宫颈腺癌细胞增殖并抑制细胞凋亡
DOI：
10.1007/s13277-015-3161-4
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发表时间：
2015-07-01
期刊：
影响因子：
--
通讯作者：
He, Mian
中科院分区：
文献类型：
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作者：
Guo, Peng;Wang, Jing;He, Mian
巨噬细胞迁移抑制因子（MIF）作为一种多功能细胞因子，与炎症和肿瘤发生有关。然而，MIF 在宫颈腺癌 (ADC) 中的作用尚不完全清楚。在本研究中，我们旨在检测MIF在ADC中的表达并探讨MIF在ADC进展中的机制。 MIF的表达与ADC的癌直径、淋巴结转移等临床病理特征呈正相关。 MIF敲低诱导ADC细胞中G1/S转变的细胞周期停滞，p21和p27表达上调，以及Cdk4、CyclinD2和CyclinE2表达下调。在MIF敲低细胞中，促凋亡蛋白Bax、caspase-3、cleaved caspase-3和cleaved-PARP表达上调，抗凋亡蛋白Bcl-2、pAkt和p53表达下调。表明MIF敲低抑制ADC细胞增殖并诱导细胞凋亡。 MIF可能是ADC诊断和治疗中的一种新型分子标志物。
As a multifunctional cytokine, macrophage migration inhibitory factor (MIF) is associated with inflammation and tumorigenesis; however, the role of MIF in cervical adenocarcinoma (ADC) is not fully understood. In this study, we aimed to examine the expression of MIF in ADC and explore the mechanism of MIF in ADC progression. MIF expression was positively related to ADC clinicopathological features of carcinoma diameter and lymph node metastasis. MIF knockdown induced cell cycle arrest of G1/S transition in ADC cells, upregulation of the expressions of p21 and p27, and downregulation of the expressions of Cdk4, CyclinD2, and CyclinE2. In MIF knockdown cells, the expressions of proapoptotic proteins of Bax, caspase-3, cleaved caspase-3, and cleaved-PARP were upregulated, and the expressions of antiapoptotic proteins of Bcl-2, pAkt, and p53 were downregulated. It indicated that MIF knockdown inhibited cell proliferation and induced apoptosis in ADC cells. MIF might be a novel molecular marker in diagnosis and therapy of ADC.