HUMAN-JK RECOMBINATION SIGNAL BINDING-PROTEIN GENE (IGKJRB) - COMPARISON WITH ITS MOUSE HOMOLOG
HUMAN-JK RECOMBINATION SIGNAL BINDING-PROTEIN GENE (IGKJRB) - COMPARISON WITH ITS MOUSE HOMOLOG
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DOI：
10.1006/geno.1993.1326
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发表时间：
1993-08-01
期刊：
影响因子：
4.4
通讯作者：
HONJO, T
中科院分区：
文献类型：
--
作者：
AMAKAWA, R;JING, W;HONJO, T
小鼠Igkjrb蛋白特异性结合免疫球蛋白Jk重组信号序列。IGKJRB基因在许多物种中高度保守，如人类、非洲爪蟾和果蝇。利用小鼠Igkjrb基因的cDNA片段，我们分离出了人类Igkjrb基因。人类基因组包含一个功能性IGKJRB基因和两种加工过的假基因。原位杂交分析表明，功能基因定位于染色体3q25，假基因（分别为IGKJRBP1和IGKJRBP2）定位于染色体9p13和9q13。该功能基因由13个外显子组成，长度至少为67 kb。通过对cDNA末端的快速扩增，分离到3种不同5′序列的cDNA，表明存在3种蛋白。aPCR-1蛋白具有外显子1序列，与小鼠RBP-2型蛋白相对应。aPCR-2和3蛋白可能是人类细胞特异性的，因为小鼠的对应蛋白未被检测到。人和小鼠IGKJRB基因的2-11外显子同源性为98%，而人和小鼠的1外显子同源性为75%。
The mouse Igkjrb protein specifically binds to the immunoglobulin Jk recombination signal sequence. The IGKJRB gene is highly conserved among many species such as human,Xenopus, andDrosophila. Using cDNA fragments of the mouse Igkjrb gene, we isolated its human counterpart, IGKJRB. The human genome contains one functional IGKJRB gene and two types of processed pseudogenes.In situchromosome hybridization analysis demonstrated that the functional gene is localized at chromosome 3q25, and the pseudogenes (IGKJRBP1 and IGKJRBP2, respectively) are located at chromosomes 9p13 and 9q13. The functional gene is composed of 13 exons spanning at least 67 kb. Three types of cDNA with different 5′ sequences were isolated by rapid amplification of cDNA ends, suggesting the presence of three proteins. The aPCR-1 protein, which possessed the exon 1 sequence, was the counterpart of the mouse RBP-2 type protein. The aPCR-2 and 3 proteins may be specific to human cells because the mouse counterparts were not detected. The amino acid sequences of the human and mouse IGKJRB genes were 98% homologous in exons 2-11, whereas the homology of the human and mouse exon 1 sequences was 75%.