Pheophytin a, a low molecular weight compound found in the marine brown alga Sargassum fulvellum, promotes the differentiation of PC12 cells
Pheophytin a, a low molecular weight compound found in the marine brown alga Sargassum fulvellum, promotes the differentiation of PC12 cells
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DOI：
10.1016/j.ijdevneu.2006.09.323
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发表时间：
2007-02-01
影响因子：
1.8
通讯作者：
Kamei, Yuto
中科院分区：
文献类型：
--
作者：
Ina, Atsutoshi;Hayashi, Ken-Ichiro;Kamei, Yuto
我们从日本海岸线收集的海洋褐藻马尾藻中鉴定并鉴定了一种神经分化化合物。多项仪器分析表明该化合物为脱镁叶绿素 a。脱镁叶绿素 a 本身并不促进 PC 12 细胞的神经突生长。然而，当 PC 12 细胞在低水平神经生长因子 (10 ng/ml) 存在下用低浓度脱镁叶绿素 a (3.9 μg/ml) 处理时，该化合物产生的神经突生长类似于高水平神经生长因子 (50 ng/ml) 产生的神经突生长。脱镁叶绿素 a 还可增强丝裂原激活蛋白激酶信号通路中的信号转导，该通路也是由神经生长因子诱导的。脱镁叶绿素 a 对 PC 12 细胞神经突生长的影响被 U0126（一种代表性的丝裂原激活蛋白激酶激酶抑制剂）完全阻断。这些结果表明，脱镁叶绿素 a 在低水平神经生长因子存在下增强 PC12 细胞的神经分化，并且这种作用是通过激活丝裂原激活蛋白激酶信号通路介导的。 (c) 2006 年 ISDN。由爱思唯尔有限公司出版。保留所有权利。
We identified and characterized a neurodifferentiation compound from the marine brown alga Sargassum fulvellum collected from the Japanese coastline. Several instrumental analyses revealed the compound to be pheophytin a. Pheophytin a did not itself promote neurite outgrowth of PC 12 cells. However, when PC 12 cells were treated with a low concentration of pheophytin a (3.9 mu g/ml) in the presence of a low level of nerve growth factor (10 ng/ml), the compound produced neurite outgrowth similar to that produced by a high level of nerve growth factor (50 ng/ml). Pheophytin a also enhanced signal transduction in the mitogen-activated protein kinase signaling pathway, which is also induced by nerve growth factor. The effect of pheophytin a on neurite outgrowth of PC 12 cells was completely blocked by U0126, a representative mitogen-activated protein kinase kinase inhibitor.These results suggest that pheophytin a enhances the neurodifferentiation of PC12 cells in the presence of a low level of nerve growth factor and that this effect is mediated by activation of a mitogen-activated protein kinase signaling pathway. (c) 2006 ISDN. Published by Elsevier Ltd. All rights reserved.