Simple and signal-off electrochemiluminescence immunosensor for alpha fetoprotein based on gold nanoparticle-modified graphite-like carbon nitride nanosheet nanohybrids
Simple and signal-off electrochemiluminescence immunosensor for alpha fetoprotein based on gold nanoparticle-modified graphite-like carbon nitride nanosheet nanohybrids
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DOI：
10.1039/c5ra26291g
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发表时间：
2016-02
期刊：
影响因子：
3.9
通讯作者：
Zheng Xiangli;Hu Xiaoxia;Qiao Xue-ying;Xia Fangquan;D. Tian;Changli Zhou
中科院分区：
文献类型：
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作者：
Zheng Xiangli;Hu Xiaoxia;Qiao Xue-ying;Xia Fangquan;D. Tian;Changli Zhou
基于金纳米粒子（AuNPs）修饰的类石墨氮化碳纳米片（g-C3 N4 NSs）纳米杂化物（Au-g-C3 N4 NHs），研制了一种简单、信号关闭的甲胎蛋白（AFP）电化学发光免疫传感器。与g-C3 N4 NS修饰玻碳电极（GCE）相比，Au-g-C3 N4 NH修饰玻碳电极的电化学发光强度更明显，这是由于AuNPs能够促进电子转移和电催化还原S2 O 82-，产生大量的空穴供体（SO 4 stec-）。结果表明，由于抗体与抗原在修饰电极上的相互作用，g-C3 N4纳米粒子的电化学发光信号减弱。因此，通过监测g-C3 N4 NS的ECL强度变化，实现了用于检测AFP的ECL免疫传感器。对影响免疫传感器性能的因素进行了详细的研究。在最佳条件下，所提出的生物传感器实现了从0.001到5.0 ng mL−1的宽线，检测限为0.0005 ng mL−1。将该电化学发光免疫传感器成功应用于血清样品中甲胎蛋白的测定，为甲胎蛋白的检测提供了一种有效的方法。
A simple, signal-off electrochemiluminescence (ECL) immunosensor for sensitive and selective detection of alpha fetoprotein (AFP) was developed based on gold nanoparticle (AuNPs) modified graphite-like carbon nitride nanosheet (g-C3N4 NSs) nanohybrids (Au-g-C3N4 NHs). Compared to the g-C3N4 NS modified glassy carbon electrode (GCE), the ECL intensity is more obvious at the Au-g-C3N4 NH modified GCE due to the fact that AuNPs can promote electron transfer and electrocatalytic reduction of S2O82− to produce large amounts of hole donor (SO4˙−). Results demonstrated that the ECL signal of the g-C3N4 NSs decreased due to the interaction between antibody and antigen on the modified electrode. Thus, an ECL immunosensor for the detection of AFP was realized by monitoring the ECL intensity change of g-C3N4 NSs. The factors influencing the performance of the immunosensor were investigated in detail. Under optimal conditions, the proposed biosensor achieved a wide line from 0.001 to 5.0 ng mL−1 with a detection limit of 0.0005 ng mL−1. Furthermore, the ECL immunosensor was successfully applied to the determination of AFP in serum samples, providing a promising effective strategy for AFP detection.