Fully linear reconstruction method for fluorescence yield and lifetime through inverse complex-source formulation: simulation studies.
Fully linear reconstruction method for fluorescence yield and lifetime through inverse complex-source formulation: simulation studies.
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DOI：
10.1364/ol.35.001899
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发表时间：
2010-06-01
期刊：
影响因子：
3.6
通讯作者：
Zhao H
中科院分区：
文献类型：
--
作者：
Gao H;Lin Y;Gulsen G;Zhao H
在荧光成像中，荧光产率和寿命都是非常重要的。传统上，利用频域数据，可以通过非线性公式直接恢复两个参数。然而，重建精度高度依赖于初始猜测。为了克服这一障碍，我们提出了通过逆复源公式的线性计划。利用频域数据的真实的和虚部，该方法是完全线性的，它是不敏感的初始猜测和高电平噪声是稳定的。同时，该算法是有效的，重建需要一个或几个迭代。此外，由于荧光成像的独特性，施加共定位约束，以提高算法的性能。用模拟数据对算法进行了测试。
In fluorescence imaging, both fluorescence yield and lifetime are of great importance. Traditionally, with the frequency-domain data, two parameters can be directly recovered through a nonlinear formulation. However, the reconstruction accuracy highly depends on initial guesses. To overcome this hurdle, we propose the linear scheme via an inverse complex-source formulation. Using the real and imaginary parts of the frequency-domain data, the proposed method is fully linear; it is not sensitive to initial guesses and is stable with high-level noise. Meanwhile, the algorithm is efficient, and the reconstruction takes one or a few iterations. In addition, the colocalization constraint due to the unique feature of fluorescence imaging is imposed to enhance algorithm performance. The algorithms are tested with simulated data.