Effect of Beauveria bassiana-Seed Treatment on Zea mays L. Response against Spodoptera frugiperda
Effect of Beauveria bassiana-Seed Treatment on Zea mays L. Response against Spodoptera frugiperda
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DOI：
10.3390/app11072887
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发表时间：
2021-04-01
影响因子：
2.7
通讯作者：
Ek-Ramos, Maria Julissa
中科院分区：
文献类型：
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作者：
Kuzhuppillymyal-Prabhakarankutty, Laiju;Ferrara-Rivero, Fernando H.;Ek-Ramos, Maria Julissa
夜蛾（Spodoptera frugiperda）是一种分布广泛的害虫，对各种作物造成重大经济损失，尤其是玉米。另一方面，球孢白僵菌是一种昆虫病原真菌，与许多植物建立共生关系，并有助于抵抗生物和非生物胁迫。在本工作中，在实验室实验中，通过种子处理将球孢白僵菌菌株GHA和一种本地菌株PTG4传递给玉米幼苗，评估了它们对frugiperda生长、发育和死亡率的影响。我们用1 × 10(6)个球孢白僵菌孢子接种玉米种子；然后这些种子在生长室条件下发芽并长成幼苗。3龄frugiperda幼虫分别以球孢白僵菌处理和未处理（阴性对照）的幼苗为食至6龄，并转入人工饲料直至成虫期。结果表明，以球孢僵菌ptg4处理过的植株为食的幼虫期延长。此外，取食用球孢白僵菌菌株处理过的植株比取食未经处理的对照植株产生较少的frugiperda雄蛾。在田间条件下，分别用1 × 10(6)个（第一次试验）和1 × 10(8)个（第二次试验）球孢白僵菌（GHA菌株）胚孢子接种玉米种子。在第一次田间试验中，阴性对照植株的幼虫数量高于球孢白僵菌处理植株。第二次田间试验中，阴性对照和球孢白僵菌处理植株均未发现幼虫。综上所述，在田间试验中，玉米球孢白僵菌种子处理降低了玉米植株果蚜的侵染。在实验室试验中，frugiperda的发育也受到影响。
Spodoptera frugiperda is a widely distributed insect pest that causes major economic losses in various crops, particularly maize. On the other hand, Beauveria bassiana is an entomopathogenic fungus that establishes symbiotic associations with many plants and contributes to tolerance against biotic and abiotic stresses. In the present work, in laboratory experiments, the effects of the B. bassiana strain GHA, in addition to a native strain (PTG4), delivered via seed treatment in maize seedlings, were evaluated on S. frugiperda growth, development, and mortality. We inoculated maize seeds with 1 x 10(6) B. bassiana blastospores; then these seeds were germinated and grown to seedlings under growth chamber conditions. Third-instar S. frugiperda larvae were allowed to feed on B. bassiana-treated and -untreated (negative control) seedlings until reaching the sixth instar and transferred to an artificial diet until reaching adult stage. Results showed that larvae feeding on B. bassiana strain PTG4-treated plants prolonged their larval stage. Furthermore, feeding on plants treated with B. bassiana strains yielded fewer S. frugiperda male moths compared with feeding with the untreated control plants. Under field conditions, 1 x 10(6) (first trial) and 1 x 10(8) (second trial) of B. bassiana (GHA strain) blastospores were used for corn seed inoculation. In the first field trial, there were a higher number of larvae in the negative control plants compared to those in the plants treated with B. bassiana. No larvae were found in negative control and B. bassiana-treated plants in the second field trial. In conclusion, seed treatment with B. bassiana in maize reduced S. frugiperda infestation of maize plants in field trials. S. frugiperda development was also affected in laboratory trials.