Surface contact inhibits neutrophil superoxide generation induced by soluble stimuli.
Surface contact inhibits neutrophil superoxide generation induced by soluble stimuli.
复制标题
表面接触抑制由可溶性刺激诱导的中性粒细胞超氧化物的产生。
DOI：
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发表时间：
1985
期刊：
影响因子：
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通讯作者：
R. Manzi
中科院分区：
文献类型：
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作者：
S. Hoffstein;D. Gennaro;R. Manzi
人体中性粒细胞在暴露于许多可溶性和颗粒性刺激时，会经历代谢爆发并产生活性氧代谢物。当与表面接触时，它们也会被刺激产生O2-。我们发现，当中性粒细胞进入被包裹的蛋白质表面时，它们产生的氧含量随蛋白质的性质而变化：IgG大于牛血清白蛋白，大于塑料，大于明胶，大于血清，大于胶原蛋白。然而，当多形核白细胞被允许停留在表面上，然后被肉豆酸酯或n -甲酰基-蛋氨酸-亮基-苯丙氨酸刺激时，与暴露于悬浮刺激的对照细胞相比，O2-反应大大减弱。相比之下，悬浮和沉淀的中性粒细胞对颗粒刺激的超氧化物产生的反应是相似的。抑制的程度与粘附的程度无关，因为在所有被测试的表面和细胞松弛素b的存在下，反应都会减弱，抑制的开始非常迅速，当细胞重新悬浮时，恢复也非常迅速。虽然O2-的产生受到表面接触的极大抑制，但溶酶体酶的释放仅受到轻微影响。表面接触的作用似乎不是通过激活腺苷酸环化酶介导的，因为磷酸二酯酶抑制剂和外源性环磷酸二丁基腺苷的结合并不能抑制磷肉豆酸酯的O2生成，但表面接触可以。这些数据表明，在渗出和趋化过程中发生的表面接触以一种未知的机制深刻地改变了中性粒细胞的行为，这意味着对悬浮中的多形核白细胞的观察不能推广到组织中的多形核白细胞。
Human neutrophils in suspension undergo a metabolic burst and generate reactive O2- metabolites upon exposure to many soluble and particulate stimuli. They can also be stimulated to produce O2- when in contact with surfaces. We found that when neutrophils were allowed to settle into protein-coated surfaces the amount of O2- they generated varied with the nature of the protein: IgG greater than bovine serum albumin greater than plastic greater than gelatin greater than serum greater than collagen. However, when polymorphonuclear leukocytes were permitted to settle onto a surface and then were stimulated with either phorbol myristate acetate or N-formyl-methionyl-leucyl-phenylalanine the O2- response was greatly diminished compared to control cells that were exposed to the stimulus in suspension. In contrast, superoxide production in response to the particulate stimulus opsonized zymosan was similar in both suspended and settled neutrophils. The degree of inhibition was not related to the degree of adherence since the diminished response occurred with all of the surfaces tested and in the presence of cytochalasin B. Onset of inhibition was very rapid as was recovery when cells were resuspended. Whereas production of O2- was greatly inhibited by surface contact, release of lysosomal enzymes was only slightly affected. The effect of surface contact did not appear to be mediated via activation of adenylate cyclase since the combination of a phosphodiesterase inhibitor and exogenous dibuteryl cyclic adenosine monophosphate did not inhibit phorbal myristate acetate O2- production, but surface contact did. These data indicate that surface contact such as would occur during diapedesis and chemotaxis profoundly alters neutrophil behavior by an unknown mechanism and imply that observations made on polymorphonuclear leukocytes in suspension cannot be generalized to polymorphonuclear leukocytes in tissue.