Microbead-based technologies in diagnostic autoantibody detection.
Microbead-based technologies in diagnostic autoantibody detection.
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DOI：
10.1517/17530050802651561
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发表时间：
2009-01-01
期刊：
影响因子：
--
通讯作者：
Fritzler, Mark L
中科院分区：
文献类型：
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作者：
Fritzler, Marvin J;Fritzler, Mark L
背景技术背景：有一个快速扩散的新技术，以确定一系列的自身抗体的医疗条件，范围从器官特异性自身免疫性疾病的全身性风湿性疾病。虽然许多实验室已经采用了高通量的诊断平台，如酶联免疫测定（ELISA），其他技术，如微珠为基础的测定正在出现作为一个替代的诊断platform.Objective：了解性能和重要性的珠为基础的免疫测定在临床诊断和治疗。使用PubMed搜索引擎结合免疫测定和Luminex关键词以及个人文献数据库对当前文献进行了综述。包括在评价和评论是基于珠的测定，如可寻址的激光珠免疫分析和相关的磁珠associations.CONCLUSIONS：与其他传统技术的比较表明，激光微珠免疫分析是可靠的，准确的，成本效益高，灵敏度高，并具有快速的周转时间的结果。虽然该诊断平台具有优势，但在常规临床诊断实验室中更广泛地接受或长期使用该技术平台之前，必须解决一些挑战。
BACKGROUND: There is a rapid proliferation of new technologies to identify a spectrum of autoantibodies in medical conditions that range from organ-specific autoimmune diseases to systemic rheumatic diseases. Although many laboratories have adopted high-throughput diagnostic platforms such as enzyme linked immunoassays (ELISA), other technologies such as microbead-based assays are emerging as an alternative diagnostic platform.OBJECTIVE: To understand the performance and importance of bead based immunoassays in clinical diagnostics and therapeutics.METHOD: Current literature was reviewed using the PubMed search engine combining keywords of immunoassay and Luminex, as well as a personal literature database. Included in the evaluation and commentary are bead-based assays such as addressable laser bead immunoassays and related magnetic bead assays.CONCLUSIONS: Comparison with other conventional technologies has indicated that laser microbead immunoassays are reliable, accurate, cost-effective, highly sensitive and have rapid turn around time for results. While there are advantages to this diagnostic platform, there are challenges that must be addressed before wider acceptance or long-term use of this technology platform in the routine clinical diagnostic laboratory.