Bio-catalysis of mango industrial waste by newly isolated Fusarium sp. (PSTF1) for pectinase production
Bio-catalysis of mango industrial waste by newly isolated Fusarium sp. (PSTF1) for pectinase production
复制标题
新分离的镰孢菌对芒果工业废物的生物催化作用。
作者：
M. Purnachandra Reddy;K. Saritha
以芒果干加工工业废弃物为碳源，对产果胶酶的真菌菌株进行了研究。从MIW中分离出8株真菌菌株，并通过果胶透明区（PCZ）技术筛选其产果胶酶的能力。镰刀菌（PSTF 1）的PCZ值最高，为52 mm。培养基的物理化学特性被标准化为高产量的果胶酶。在温度-28 °C，pH-6.0，接种量0.6/25 ml，培养-72 h，底物浓度-0.6 g/100 ml，碳源-果糖（1%）下观察到深层发酵中果胶酶的最高产量。对不同氨基酸、维生素的效果也进行了观察。在此最佳条件下，观察到最高酶活81.9718 µmol/ml。镰刀菌PSTF 1是产果胶酶最好的菌株。以廉价的废弃物为碳源，高产高附加值果胶酶。
The dried mango fruit processing industrial waste (MIW) used as carbon source for the production of pectinase from fungal strains. Eight fungal strains were isolated from MIW and screened for their ability to produce pectinase by pectin clear zone (PCZ) technique. Fusarium sp. (PSTF1) showed highest PCZ value of 52 mm. The physico-chemical characteristics of the medium were standardized for high production of pectinase. The highest production of pectinase in submerged fermentation observed at temperature—28 °C, pH-6.0, inoculum-size 0.6/25 ml, incubation—72 h, substrate concentration—0.6 g/100 ml, carbon source-fructose (1 %). The effect of different amino acids, vitamins also observed. Under these optimal conditions the highest activity 81.9718 µmol/ml of pectinase was observed. The Fusarium sp. (PSTF1) has been considered as the best pectinase producer in submerged fermentation of MIW. The cheap waste raw material used as best carbon source for high production of high value pectinase.