Usefulness of IgG4 subclass antibodies for diagnosis of human clonorchiasis
Usefulness of IgG4 subclass antibodies for diagnosis of human clonorchiasis
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DOI：
10.3347/kjp.1999.37.4.243
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发表时间：
1999-12-01
影响因子：
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通讯作者：
Lee, Soon-Hyung
中科院分区：
文献类型：
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作者：
Hong, Sung-Tae;Lee, Mejeong;Lee, Soon-Hyung
本研究分析了华支睾吸虫主要抗原带的血清 IgG 亚类抗体反应，以探讨其血清诊断的改进。 4个亚类抗体中，产生了IgG1和IgG2抗体，但不具有特异性，IgG3抗体产生最少，IgG4抗体突出且具有特异性。 168例卵子阳性病例中，65.5%的患者血清中出现43-50、34-37、26-28和8 kDa条带的IgG抗体反应，其中22.0%的患者出现IgG4抗体反应。 IgG和IgG4抗体的阳性率与感染强度直接相关。 EPG 5000以上的重度感染病例的所有血清均呈特异性IgG和IgG4抗体阳性反应。治疗后6个月内特异性血清IgG4抗体消失。 35 kDa 和 67 kDa 的条带与 IgG 抗体发生交叉反应，但不与其他吸虫感染血清中的 IgG4 抗体发生交叉反应。目前的研究结果表明，血清 IgG4 抗体对 8 kDa 条带的反应是特异性的，但不敏感。需要任何提高其敏感性的方法来改进血清诊断。
The present study analyzed serum IgG subclass antibody reaction to major antigenic bands of Clonorchis sinensis to investigate improvement of its serodiagnosis. Of the four subclass antibodies, IgG1 and IgG2 antibodies were produced but not specific, IgG3 antibody was least produced, and IgG4 antibody was prominent and specific. The serum IgG antibody reaction to any of 43-50, 34-37, 26-28, and 8 kDa bands was found in 65.5% of 168 egg positive cases while IgG4 antibody reaction was found in 22.0% of them. The positive rates of IgG and IgG4 antibodies were directly correlated with the intensity of infection. All of the sera from heavily infected cases over EPG 5,000 showed positive reaction for specific IgG and IgG4 antibodies. The specific serum IgG4 antibody disappeared within 6 months after treatment. The bands of 35 kDa and 67 kDa cross-reacted with IgG antibodies but not with IgG4 antibodies in sera of other trematode infections. The present findings suggest that serum IgG4 antibody reaction to 8 kDa band is specific but not sensitive. Any method to increase its sensitivity is required for improved serodiagnosis.