Huangqi-Honghua combination and its main components ameliorate cerebral infarction with Qi deficiency and blood stasis syndrome by antioxidant action in rats
Huangqi-Honghua combination and its main components ameliorate cerebral infarction with Qi deficiency and blood stasis syndrome by antioxidant action in rats
复制标题
黄芪红花组合及其主要成分通过抗氧化作用改善脑梗塞气虚血瘀证。
DOI：
10.1016/j.jep.2014.05.061
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发表时间：
2014-09-11
影响因子：
5.4
通讯作者：
Wen, Aidong
中科院分区：
文献类型：
--
作者：
Cao, Jinyi;Chen, Zhengyu;Wen, Aidong
民族药理学相关性：黄芪红花组合在中国作为传统草药广泛用于治疗中风和心肌缺血性疾病的气虚血瘀证（QDBS）。目的：探讨黄芪红花组合（HH）及其主要成分黄芪甲苷IV（AS-IV）和羟基红花黄色素A（HSYA）的疗效材料与方法：雄性大鼠随机分为六组：假手术组、QDBS+I/R模型组和AS-IV、HSYA、AS-IV+HSYA和HH治疗组。再灌注24小时后评估全血粘度（WBV）、血浆粘度（PV）、神经系统检查、梗死体积、组织病理学变化和一些氧化应激标志物。结果：HH及其主要成分AS-IV+HSYA可显着降低再灌注24小时后的WBV、PV，并显着改善神经系统检查和梗死体积。它们还显着增加核因子红细胞 2 相关因子 2 (Nrf2) 的表达，超氧化物歧化酶 (SOD)、过氧化氢酶和谷胱甘肽过氧化物酶 (GSH-Px) 等抗氧化剂的活性，导致丙二醛 (MDA) 和活性氧 (ROS) 水平降低。结论：AS-IV 和 HSYA 是 HH 的主要疗效的原因。该研究为进一步了解HH及其主要成分祛瘀、改善脑梗塞的作用机制提供科学依据。此外，AS-IV 和 HSYA 似乎对神经保护具有协同作用。 (C) 2014 Elsevier Ireland Ltd. 保留所有权利。
Ethnopharmacological relevance: Combination of Radix Astragali (Huangqi) and Carthamus tinctorius L (Honghua) has been extensively used as traditional herb medicine in China for the treatment of stroke and myocardial ischemia diseases with Qi deficiency and blood stasis (QDBS) syndrome.Aim: To investigate the effect of Huangqi Honghua combination (HH) and its main components astragaloside IV (AS-IV) and Hydroxysafflor yellow A (HSYA) on cerebral ischemia-reperfusion (IR) with QDBS in rat model.Materials and methods: Male rats were randomly divided into the following six groups: sham group, QDBS+I/R model group and treatment group including AS-IV, HSYA, AS-IV+HSYA and HH. The whole blood viscosity (WBV), plasma viscosity (PV), neurological examination, infarct volume, histopathology changes and some oxidative stress markers were assessed after 24 h of reperfusion.Results: HH and its main components AS-IV+HSYA could significantly decrease WBV, PV, and also significantly ameliorate neurological examination and infarct volume after 24 h of reperfusion. They also significantly increased expression of Nuclear factor erythroid 2-related factor 2 (Nrf2), activities of antioxidants, such as superoxide dismutase (SOD), catalase and glutathione peroxidase (GSH-Px), led to decrease levels of malondialdehyde (MDA) and reactive oxygen species (ROS).Conclusion: AS-IV and HSYA are responsible for the main curative effects of HH. The study may provide scientific information to further understanding the mechanism(s) of HH and its main components in removing blood stasis and ameliorating cerebral infarction. Additionally, AS-IV and HSYA appear to have synergistic effects on neuroprotection. (C) 2014 Elsevier Ireland Ltd. All rights reserved.