Rapid immunohistochemical detection of Rhodococcus equi in impression smears from affected foals on postmortem examination.
Rapid immunohistochemical detection of Rhodococcus equi in impression smears from affected foals on postmortem examination.
复制标题
尸检时，对受影响马驹的印迹涂片中的马红球菌进行快速免疫组织化学检测。
DOI：
10.1046/j.1439-0450.2001.00505.x
复制
发表时间：
2001
期刊：
影响因子：
--
通讯作者：
B. Dénes
中科院分区：
文献类型：
--
作者：
L. Szeredi;L. Makrai;B. Dénes
本研究的第一个目的是发展一种免疫组化方法，用于快速检测马红球菌在马驹死后检查受影响器官的印模涂片。第二个目的是证明R.使用免疫组织化学方法，可以在从相同马驹收集的气管分泌物的涂片中检测到马。对3只死于呼吸道感染的马驹（A、B和C）的肺和纵隔淋巴结进行了涂片、冰冻切片和石蜡包埋切片。马，也来自马驹A的肠系膜尾淋巴结。从所有马驹的气管分泌物中制备印模涂片。用亲和纯化的兔IgG进行免疫组化显示。等。该抗体可与血清1型红曲霉反应。在Ouchterlony免疫扩散和被动血凝试验中，马链球菌与其他血清型或马链球菌亚种不相关。牛分枝杆菌、马分枝杆菌、金黄色葡萄球菌免疫组化反应阴性。副结核病免疫组化方法对细菌培养的敏感性相同，而且，从一匹马驹的肺和纵隔淋巴结中，发现了R。Equi只能通过该方法检测。R.马在所有三匹马驹的气管渗出物涂片中得到证实。本研究结果表明，免疫组化方法可用于快速检测红细胞癌。马在受影响的器官，特别是死后获得的脓肿的印迹涂片中，可能作为诊断R.通过检查气管吸出物涂片，在活马驹中发现马肺炎。
The first objective of this study was to develop an immunohistochemical procedure for rapid detection of Rhodococcus equi in impression smears from affected organs of foals on postmortem examination. The second aim was to demonstrate whether R. equi can be detected in smears of tracheal exudates collected from the same foals using an immunohistochemical method. Impression smears and cryostat and paraffin-embedded sections were made from the lungs and mediastinal lymph nodes of three foals (A, B and C) that had died of respiratory disease caused by R. equi, and also from the caudal mesenteric lymph node of foal A. Impression smears were made from the tracheal exudates of all foals. An affinity purified rabbit IgG was used for the immunohistochemical demonstration of R. equi. This antibody reacted with serotype 1 of R. equi in Ouchterlony's immunodiffusion and in the passive haemagglutination test, but not with other serotypes or with Streptococcus equi ssp. equi or Staphylococcus aureus, and failed to give an immunohistochemical reaction with Mycobacterium bovis or M. paratuberculosis. The immunohistochemical method proved to be of identical sensitivity to bacterial culture; moreover, from the lungs and mediastinal lymph nodes of one foal, R. equi could only be detected by this method. R. equi was demonstrated in smears of the tracheal exudates of all three foals. The results of this study indicate that the immunohistochemical method may be used for the rapid detection of R. equi in impression smears from the affected organs, especially abscesses, obtained postmortem, and possibly as a tool for diagnosing R. equi pneumonia in live foals by examining smears of tracheal aspirates.