Identification of two additional plasmodesmata localization domains in the tobacco mosaic virus cell-to-cell-movement protein
Identification of two additional plasmodesmata localization domains in the tobacco mosaic virus cell-to-cell-movement protein
复制标题
烟草花叶病毒细胞间运动蛋白中两个额外胞间连丝定位域的鉴定
DOI：
10.1016/j.bbrc.2019.10.093
复制
发表时间：
2020-01-01
影响因子：
3.1
通讯作者：
Yuan, Cheng
中科院分区：
文献类型：
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作者：
Liu, Yong;Huang, Changjun;Yuan, Cheng
尽管数十年的深入研究，未能确定胞间连丝（PD）定位序列限制了我们对烟草花叶病毒（TMV）运动的理解。最近，我们确定了第一个PD定位信号（主要PLS）在TMV运动蛋白（MP），其中包括前50个氨基酸残基的MP。虽然主要PLS足以用于PD靶向，但效率低于全长TMV MP。为了解决这一效率差距，我们鉴定了两个额外的PLS结构域，包括MP的氨基酸残基61至80和147至170，并显示这两个结构域靶向PD，但不转运至相邻细胞。我们还证明了MP61 - 80片段与拟南芥突触结合蛋白A相互作用，该蛋白A也与主要的TMV MP PLS相互作用。因此，我们的研究结果提供了新的见解，以更全面地了解潜在的TMV MP的胞间连丝靶向的机制。(C)2019爱思唯尔公司All rights reserved.
Despite decades of intensive studies, the failure to identify plasmodesmata (PD) localization sequences has constrained our understanding of Tobacco mosaic virus (TMV) movement. Recently, we identified the first PD localization signal (major PLS) in the TMV movement protein (MP), which encompasses the first 50 amino acid residues of the MP. Although the major PLS is sufficient for PD targeting, the efficiency is lower than the full-length TMV MP. To address this efficiency gap, we identified two additional PLS domains encompassing amino acid residues 61 to 80, and 147 to 170 of the MP and showed that these two domains target to PD, but do not transit to adjacent cells. We also demonstrated that the MP61-80 fragment interacts with Arabidopsis synaptotagmin A, which was also shown to interact with the major TMV MP PLS. Therefore, our findings have provided new insights to more fully understand the mechanism underlying plasmodesmal targeting of TMV MP. (C) 2019 Elsevier Inc. All rights reserved.