Topical application of dressing with amino acids improves cutaneous wound healing in aged rats
Topical application of dressing with amino acids improves cutaneous wound healing in aged rats
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DOI：
10.1016/j.acthis.2009.05.003
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发表时间：
2010-01-01
影响因子：
2.5
通讯作者：
Rezzani, Rita
中科院分区：
文献类型：
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作者：
Corsetti, Giovanni;D'Antona, Giuseppe;Rezzani, Rita
治疗手术和非手术伤口，特别是老化皮肤的主要目标是需要快速闭合病变。皮肤伤口愈合过程涉及四个阶段，包括诱导促炎细胞因子的炎症反应。如果细菌感染引起炎症，可能会对伤口闭合造成问题。减少炎症加速伤口闭合，随后增加成纤维细胞功能和胶原蛋白合成。相反，长期慢性炎症导致非常有限的伤口愈合。使用组织学和免疫组织化学技术，我们研究了一种名为Vulnamin（R）的新伤口敷料（含有胶原蛋白和弹性蛋白合成所必需的四种氨基酸）加伊曲糖酸钠（Na-lal）与单独Na-lal相比在老年大鼠实验性皮肤伤口闭合中的作用。我们的结果表明，Vulnamin敷料的应用通过减少炎性细胞数量和诱导型一氧化氮合酶（iNOS）免疫定位来调节炎症反应，同时增加内皮型一氧化氮合酶（eNOS）和转化生长因子-β 1（TGF-β 1）免疫定位。此外，敷料增加了成纤维细胞的分布密度，并有助于合成薄胶原纤维，从而缩短愈合时间。使用这种新型伤口敷料的营养方法可以提供一种有效且安全的策略，以加速老年受试者的伤口愈合，简化治疗程序并改善生活质量。(C)2009年Elsevier GmbH。All rights reserved.
The principal goal in treating surgical and non-surgical wounds, in particular for aged skin, is the need for rapid closure of the lesion. Cutaneous wound healing processes involve four phases including an inflammatory response with the induction of pro-inflammatory cytokines. If inflammation develops in response to bacterial infection, it can create a problem for wound closure. Reduced inflammation accelerates wound closure with subsequent increased fibroblast function and collagen synthesis. On the contrary, prolonged chronic inflammation results in very limited wound healing. Using histological and immunohistochemical techniques, we investigated the effects of a new wound dressing called Vulnamin (R) that contains four essential amino acids for collagen and elastin synthesis plus sodium ialuronate (Na-lal), compared with Na-lal alone, in closure of experimental cutaneous wounds of aged rats. Our results showed that the application of Vulnamin dressings modulated the inflammatory response with a reduction in the number of inflammatory cells and inducible nitric oxide synthase (iNOS) immunolocalisation, while increasing endothelial nitric oxide synthase (eNOS) and transforming growth factor-beta 1 (TGF-beta 1) immunolocalisation. Furthermore, the dressing increased the distribution density of fibroblasts and aided the synthesis of thin collagen fibers resulting in a reduction in healing time. The nutritive approach using this new wound dressing can provide an efficacious and safe strategy to accelerate wound healing in elderly subjects, simplifying therapeutic procedures and leading to an improved quality of life. (C) 2009 Elsevier GmbH. All rights reserved.