Wnt signaling and PKA control Nodal expression and left-right determination in the chick embryo.
Wnt signaling and PKA control Nodal expression and left-right determination in the chick embryo.
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发表时间：
2001-08
期刊：
影响因子：
4.6
通讯作者：
Concepción Rodríguez-Esteban;J. Capdevila;Y. Kawakami;J. C. I. Belmonte
中科院分区：
文献类型：
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作者：
Concepción Rodríguez-Esteban;J. Capdevila;Y. Kawakami;J. C. I. Belmonte
Nodal基因编码分泌因子的TGF β超家族成员，其表达定位于迄今为止检查的所有脊椎动物中发育胚胎的左侧。这种不对称模式与左右轴的正常发育相关。我们现在表明，Wnt和PKA信号通路控制左，右决定在鸡胚通过Nodal。Wnt/β-连环蛋白途径控制Hensen结中及其周围的Nodal表达，而不影响上游调节因子Sonic hedgehog、Car和成纤维细胞生长因子8。Nodal的转录也受到蛋白激酶A依赖性途径的正调控。粘附蛋白N-cadherin和PKI（一种内源性蛋白激酶A抑制剂）都定位于结的右侧，并且可能有助于通过Wnt信号传导和PKA将Nodal激活限制在结的左侧。
Expression of the Nodal gene, which encodes a member of the TGFbeta superfamily of secreted factors, localizes to the left side of the developing embryo in all vertebrates examined so far. This asymmetric pattern correlates with normal development of the left-right axis. We now show that the Wnt and PKA signaling pathways control left-right determination in the chick embryo through Nodal. A Wnt/beta-catenin pathway controls Nodal expression in and around Hensen's node, without affecting the upstream regulators Sonic hedgehog, Car and Fibroblast Growth Factor 8. Transcription of Nodal is also positively regulated by a protein kinase A-dependent pathway. Both the adhesion protein N-cadherin and PKI (an endogenous protein kinase A inhibitor) are localized to the right side of the node and may contribute to restrict Nodal activation by Wnt signaling and PKA to the left side of the node.