Rab11 is required for membrane trafficking and actomyosin ring constriction in meiotic cytokinesis of Drosophila males
Rab11 is required for membrane trafficking and actomyosin ring constriction in meiotic cytokinesis of Drosophila males
复制标题
在果蝇雄体减数分裂过程中，膜贩运和肌动蛋白环收缩需要 Rab11
DOI：
10.1091/mbc.e07-05-0415
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发表时间：
2007-12-01
影响因子：
3.3
通讯作者：
Gatti, Maurizio
中科院分区：
文献类型：
--
作者：
Giansanti, Maria Grazia;Belloni, Giorgio;Gatti, Maurizio
Rab11 是一种小型 GTP 酶，可调节囊泡运输的多个方面。在这里，我们证明 Rab11 在果蝇精母细胞的卵裂沟处积累，并且它对于胞质分裂至关重要。突变的精母细胞形成规则的肌动球蛋白环，但这些环无法收缩完成，导致胞质分裂失败。 rab11 精母细胞还在末期赤道处表现出高尔基体衍生囊泡的异常积累，表明膜囊泡融合存在缺陷。这些胞质分裂表型与 giotto (gio) 和四轮驱动 (fwd) 突变引起的表型相同，分别编码磷脂酰肌醇转移蛋白和磷脂酰肌醇 4-激酶。 Gio和Rab11的双突变体分析和免疫染色表明，gio、fwd和rab11在相同的细胞因子途径中发挥作用，其中Gio和Fwd作用于Rab11的上游。我们建议 Gio 和 Fwd 介导 Rab11 在卵裂沟处的募集，并且 Rab11 促进靶向膜递送至前进的沟。
Rab11 is a small GTPase that regulates several aspects of vesicular trafficking. Here, we show that Rab11 accumulates at the cleavage furrow of Drosophila spermatocytes and that it is essential for cytokinesis. Mutant spermatocytes form regular actomyosin rings, but these rings fail to constrict to completion, leading to cytokinesis failures. rab11 spermatocytes also exhibit an abnormal accumulation of Golgi-derived vesicles at the telophase equator, suggesting a defect in membrane-vesicle fusion. These cytokinesis phenotypes are identical to those elicited by mutations in giotto (gio) and four wheel drive (fwd) that encode a phosphatidylinositol transfer protein and a phosphatidylinositol 4-kinase, respectively. Double mutant analysis and immunostaining for Gio and Rab11 indicated that gio, fwd, and rab11 function in the same cytokinetic pathway, with Gio and Fwd acting upstream of Rab11. We propose that Gio and Fwd mediate Rab11 recruitment at the cleavage furrow and that Rab11 facilitates targeted membrane delivery to the advancing furrow.