Bacterial assay of contact lens wearers
Bacterial assay of contact lens wearers
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DOI：
10.1097/00006324-199603000-00014
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发表时间：
1996-03-01
影响因子：
1.4
通讯作者：
Farris, RL
中科院分区：
文献类型：
--
作者：
Hart, DE;Hosmer, M;Farris, RL
目的。该项目的目标是确定隐形眼镜和眼睛邻近区域的细菌数量。本文对日常佩戴和长期佩戴一次性隐形眼镜的混合人群的隐形眼镜和眼部需氧菌群进行了定量研究。方法：研究方法。对隐形眼镜、下穹隆、下穹隆收集的泪液和眉板腺边缘的下睑边缘进行细菌集落形成单位(CFU)的测定。对18例配戴一次性高含水率水凝胶隐形眼镜的18例患者进行检测，平均晶状体年龄为8.8±4.6天。3名患者每天配戴隐形眼镜，15名患者延长配戴时间表。用无菌微生物环获取泪液样本，并用组织研磨机将晶状体浸泡成小颗粒。将样品倒入巧克力琼脂平板表面，在35摄氏度的5%二氧化碳中孵化48小时。结果。眼睑边缘细菌最多(平均值为9.7 CFU；中位数为2 CFU；MODE为0 CFU)。晶状体中CFU的出现位居第二(平均4.5 CFU；中位数=1 CFU；模式=0)。穹隆和泪液细菌检出最少(穹窿：平均值=2.6CFU；中位数=0CFU；模式=0CFU)(泪液：平均值=1.6CFU；中位数=0CFU；模式=0CFU)。细菌为凝固酶阴性葡萄球菌。结论。对佩戴一次性隐形眼镜的受试者进行的细菌检测表明，眼盖边缘是最大的细菌来源，泪水是最低的。这些研究支持这样一个概念，即在正常情况下，眼睛的晶状体通常不会有大量细菌。
Purpose. The goal of the project was to determine the quantity of bacteria on the contact lens and adjacent areas of the eye. This paper is a quantitative study of the contact lens and ocular aerobic microbiota in a mixed group of daily and extended wear disposable contact lens users. Methods. The contact lens, the lower fornix, tears collecting at the lower fornix, and edge of the lower lid at the Meibomian gland margin were assayed for the quantity of bacterial colony forming units (CFU). Eighteen patients wearing 49 disposable high water content hydrogel contact lenses were assayed and the mean lens age was 8.8 +/- 4.6 days. Three patients wore their lenses on a daily wear basis and 15 on an extended wear schedule. Tear samples were obtained with sterile microbial loops and the lens was macerated into small particles with a tissue grinder. The samples were poured onto the surface of chocolate agar plates and incubated at 35 degrees C for 48 h in 5% CO2. Results. The lid margin revealed the greatest bacterial presence (mean = 9.7 CFU; median = 2 CFU; mode = 0 CFU). The lens showed the next greatest presence of CFU (mean 4.5 CFU; median = 1 CFU; mode = 0). The fornix and tears revealed the least bacterial presence (fornix: mean = 2.6 CFU; median = 0 CFU; mode = 0 CFU) (tears: mean = 1.6 CFU; median = 0 CFU; mode = 0 CFU). The bacteria were coagulase-negative staphylococci. Conclusions. The bacterial assay of disposable lens wearing contact lens subjects indicates that the lid margins are the greatest source of bacteria with the tears being the lowest. These studies support the concept that in the eye, the lens typically does not possess a large number of bacteria under normal conditions.