THE ROLE OF CARBOHYDRATE IN ERYTHROPOIETIN ACTION
THE ROLE OF CARBOHYDRATE IN ERYTHROPOIETIN ACTION
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DOI：
10.1210/endo-116-6-2293
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发表时间：
1985-01-01
期刊：
影响因子：
4.8
通讯作者：
GOLDWASSER, E
中科院分区：
文献类型：
--
作者：
DORDAL, MS;WANG, FF;GOLDWASSER, E
用微量GLC法测定了人促红细胞生成素（epo）的糖组成。酶法去除大部分糖导致糖苷酶处理的epo聚集，在小鼠中测定时生物活性丧失，在骨髓细胞培养物中或通过RIA [放射免疫测定法]测定时活性保留。内切糖苷酶F导致去除大部分碳水化合物，表明寡糖是天冬酰胺连接的。通过不存在N-乙酰半乳糖胺证实了O-连接糖的缺乏。显然，epo的寡糖部分，虽然是体内作用所必需的，但并不是与造血系统靶细胞相互作用所必需的。
The carbohydrate composition of human erythropoietin (epo) was determined by micro-GLC. Enzymic removal of most of the sugars results in aggregation of glycosidase-treated epo, loss of biological activity when assayed in mice, and retention of activity when assayed in marrow cell cultures or by RIA [radioimmunoassay]. Endoglycosidase F causes the removal of most of the carbohydrates, indicating that the oligosaccharides are asparagine linked. The lack of O-linked sugar is confirmed by the absence of N-acetylgalactosamine. Apparently, the oligosaccharide portion of epo, although required for action in vivo, is not required for interaction with the target cells of the bloodforming system.