Lipidomic analysis of phospholipids from human mammary epithelial and breast cancer cell lines
Lipidomic analysis of phospholipids from human mammary epithelial and breast cancer cell lines
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DOI：
10.1002/jcp.24152
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发表时间：
2013-02-01
影响因子：
5.6
通讯作者：
Helguero, Luisa A.
中科院分区：
文献类型：
--
作者：
Luisa Doria, M.;Cotrim, Candida Z.;Helguero, Luisa A.
磷脂（PL）谱的改变已与疾病和特定的脂质可能参与癌症的发病和演变，然而，在乳腺癌细胞中使用质谱（MS）的PL谱分析是一种新的方法。以前，我们报告了脂质组学分析PL从小鼠乳腺上皮细胞和乳腺癌细胞使用离线薄层色谱（TLC）-MS，其中PL配置文件的几个变化被发现与恶性程度的细胞。在本研究中，脂质组学分析已扩展到人乳腺上皮细胞和乳腺癌细胞系（MCF 10A、T47-D和MDA-MB-231），使用TLC-MS，通过亲水相互作用液相色谱-MS验证。磷脂酰乙醇胺（PE）含量相对于PL总量的差异在非恶性细胞中磷脂酸的相对丰度最高，而转移性细胞中磷脂酸的相对丰度最高。此外，发现与癌症表型、转移潜力和细胞形态相关的PL分子种类存在以下差异：在迁移细胞中检测到较高水平的烷基酰基PC和磷脂酰肌醇（PI; 22：5/18：0），上皮细胞在PE和鞘磷脂类中具有较少的不饱和脂肪酰基链和较短的脂肪族尾部，而与癌细胞相比，PI（18：0/18：1）在非恶性细胞中最低。到目前为止，有关PL在癌症进展中的变化的信息是稀缺的，因此，这项工作中提出的结果将是有用的起点，以定义可能的PL与前瞻性的生物标志物，并披露代谢途径与癌症治疗的潜力。J.细胞。228：457468，2013。(c)2012 Wiley Periodicals，Inc.
Alterations of phospholipid (PL) profiles have been associated to disease and specific lipids may be involved in the onset and evolution of cancer; yet, analysis of PL profiles using mass spectrometry (MS) in breast cancer cells is a novel approach. Previously, we reported a lipidomic analysis of PLs from mouse mammary epithelial and breast cancer cells using off-line thin layer chromatography (TLC)-MS, where several changes in PL profile were found to be associated with the degree of malignancy of cells. In the present study, lipidomic analysis has been extended to human mammary epithelial cells and breast cancer cell lines (MCF10A, T47-D, and MDA-MB-231), using TLC-MS, validated by hydrophilic interaction liquid chromatography-MS. Differences in phosphatidylethanolamine (PE) content relative to total amount of PLs was highest in non-malignant cells while phosphatidic acid was present with highest relative abundance in metastatic cells. In addition, the following differences in PL molecular species associated to cancer phenotype, metastatic potential, and cell morphology were found: higher levels of alkylacyl PCs and phosphatidylinositol (PI; 22:5/18:0) were detected in migratory cells, epithelial cells had less unsaturated fatty acyl chains and shorter aliphatic tails in PE and sphingomyelin classes, while PI (18:0/18:1) was lowest in non-malignant cells compared to cancer cells. To date, information about PL changes in cancer progression is scarce, therefore results presented in this work will be useful as a starting point to define possible PLs with prospective as biomarkers and disclose metabolic pathways with potential for cancer therapy. J. Cell. Physiol. 228: 457468, 2013. (c) 2012 Wiley Periodicals, Inc.