Hypoxia directed migration of human naïve monocytes is associated with an attenuation of cytokine release: indications for a key role of CCL26.
Hypoxia directed migration of human naïve monocytes is associated with an attenuation of cytokine release: indications for a key role of CCL26.
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DOI：
10.1186/s12967-020-02567-7
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发表时间：
2020-10-21
影响因子：
7.4
通讯作者：
Zitta K
中科院分区：
文献类型：
--
作者：
Hummitzsch L;Berndt R;Kott M;Rusch R;Faendrich F;Gruenewald M;Steinfath M;Albrecht M;Zitta K
许多组织来源的因子已被假定参与循环单核细胞的组织迁移。本研究的目的是评估是否定义的缺氧梯度可以诱导幼稚的人单核细胞定向迁移，并确定负责的自分泌/旁分泌因子。从外周血单核细胞中分离单核细胞，转移到趋化性室中，并在添加或不添加CCL 26的情况下经受限定的氧梯度。记录细胞迁移并进行分泌组分析。细胞迁移记录显示，定向迁移的单核细胞对缺氧源。对单核细胞分泌组的分析表明，在缺氧条件下，70%（19/27）的所分析的细胞因子的分泌减少。下调最多的因子是CCL 26（-99%），CCL 1（-95%），CX 3CL 1（-95%），CCL 17（-85%）和XCL 1（-83%）。重组CCL 26的管理废除了缺氧诱导的人单核细胞的定向迁移，而在常氧条件下添加CCL 26导致单核细胞从CCL 26的来源排斥。缺氧诱导体外人单核细胞定向迁移。自分泌/旁分泌释放的CCL 26参与了缺氧介导的单核细胞迁移，并可能代表调节体内单核细胞迁移的靶分子。
Numerous tissue-derived factors have been postulated to be involved in tissue migration of circulating monocytes. The aim of this study was to evaluate whether a defined hypoxic gradient can induce directed migration of naïve human monocytes and to identify responsible autocrine/paracrine factors. Monocytes were isolated from peripheral blood mononuclear cells, transferred into chemotaxis chambers and subjected to a defined oxygen gradient with or without the addition of CCL26. Cell migration was recorded and secretome analyses were performed. Cell migration recordings revealed directed migration of monocytes towards the source of hypoxia. Analysis of the monocyte secretome demonstrated a reduced secretion of 70% (19/27) of the analyzed cytokines under hypoxic conditions. The most down-regulated factors were CCL26 (− 99%), CCL1 (− 95%), CX3CL1 (− 95%), CCL17 (− 85%) and XCL1 (− 83%). Administration of recombinant CCL26 abolished the hypoxia-induced directed migration of human monocytes, while the addition of CCL26 under normoxic conditions resulted in a repulsion of monocytes from the source of CCL26. Hypoxia induces directed migration of human monocytes in-vitro. Autocrine/paracrine released CCL26 is involved in the hypoxia-mediated monocyte migration and may represent a target molecule for the modulation of monocyte migration in-vivo.
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影响因子：
4.3
作者：
Huang Y;Zitta K;Bein B;Steinfath M;Albrecht M
通讯作者：
Albrecht M
影响因子：
15.9
作者：
SCHMIDT, AM;YAN, SD;STERN, D
通讯作者：
STERN, D
影响因子：
5.6
作者：
Sleeboom JJF;Toonder JMJD;Sahlgren CM
通讯作者：
Sahlgren CM
影响因子：
4.4
作者：
Bosco, Maria Carla;Puppo, Maura;Varesio, Luigi
通讯作者：
Varesio, Luigi
影响因子：
20.3
作者：
Ogilvie, P;Paoletti, S;Uguccioni, M
通讯作者：
Uguccioni, M