Conservation of Dynamics Associated with Biological Function in an Enzyme Superfamily.
Conservation of Dynamics Associated with Biological Function in an Enzyme Superfamily.
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DOI：
10.1016/j.str.2018.01.015
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发表时间：
2018-03-06
期刊：
影响因子：
--
通讯作者：
Agarwal PK
中科院分区：
文献类型：
--
作者：
Narayanan C;Bernard DN;Bafna K;Gagné D;Chennubhotla CS;Doucet N;Agarwal PK
具有共同化学和/或生物功能的酶超家族成员也有共同的特征。虽然结构的作用得到了很好的描述，但酶功能和动力学之间的联系还没有被很好地理解。我们系统地描述了胰型核糖核酸酶(RNase)超家族的二十多个成员的内在动力学特征，这些成员共享一个共同的结构折叠。这项研究的动机是这样一个事实，即核糖核酸酶同系物的化学活性和分子运动范围跨越105倍以上。动力学是通过核磁共振实验和计算机模拟相结合的方式来表征的。系统发育聚类导致将序列分成功能不同的亚家族。对各种核糖核酸酶的详细描述表明，亚家族中的酶具有保守的动力学特征。这些结果表明，动力学行为保守的选择性压力，以及其他因素，可能与酶超家族中不同的化学和生物功能有关。胰型核糖核酸酶超家族包含多种生物学功能的酶，包括血管生成、宿主防御和核糖核溶解活性。Narayanan，Bernard等人。证明该家族的成员可以被划分为功能不同的亚家族，具有保守的动态特征，这可能与其特定的功能有关。
Enzyme superfamily members that share common chemical and/or biological functions also share common features. While the role of structure is well characterized, the link between enzyme function and dynamics is not well understood. We present a systematic characterization of intrinsic dynamics of over twenty members of the pancreatic-type ribonuclease (RNase) superfamily, which share a common structural fold. This study is motivated by the fact that the range of chemical activity as well as molecular motions of RNase homologs span over 105-folds. Dynamics was characterized using a combination of nuclear magnetic resonance experiments and computer simulations. Phylogenetic clustering led to the grouping of sequences into functionally distinct sub-families. Detailed characterization of the diverse RNases showed conserved dynamical traits for enzymes within sub-families. These results suggest that selective pressure for the conservation of dynamical behavior, among other factors, may be linked to the distinct chemical and biological functions in an enzyme superfamily. The pancreatic-type ribonuclease enzyme superfamily contains enzymes with diverse biological functions including angiogenesis, host defense and ribonucleolytic activity. Narayanan, Bernard et al. demonstrate that members of the family can be classified into functionally distinct subfamilies with conserved dynamical traits, which may be linked to their specific functions.
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DOI：
10.1093/protein/4.7.831
发表时间：
1991-10-01
期刊：
PROTEIN ENGINEERING
影响因子：
--
作者：
ALLEMANN, RK;PRESNELL, SR;BENNER, SA
通讯作者：
BENNER, SA
影响因子：
56.9
作者：
Boehr, David D.;McElheny, Dan;Wright, Peter E.
通讯作者：
Wright, Peter E.
影响因子：
64.8
作者：
Eisenmesser, EZ;Millet, O;Kern, D
通讯作者：
Kern, D
影响因子：
15
作者：
Agarwal, PK
通讯作者：
Agarwal, PK
影响因子：
14.9
作者：
Domachowske, JB;Dyer, KD;Rosenberg, HF
通讯作者：
Rosenberg, HF