Longitudinal shift in diabetic wound microbiota correlates with prolonged skin defense response
Longitudinal shift in diabetic wound microbiota correlates with prolonged skin defense response
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DOI：
10.1073/pnas.1004204107
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发表时间：
2010-08-17
影响因子：
11.1
通讯作者：
Segre, Julia A.
中科院分区：
文献类型：
--
作者：
Grice, Elizabeth A.;Snitkin, Evan S.;Segre, Julia A.
糖尿病患者经常患有慢性、不愈合的伤口。虽然人们普遍认为细菌定植和/或感染会对伤口愈合产生负面影响，但微生物群落与伤口愈合受损之间的确切关系尚不清楚。由于宿主皮肤防御反应被认为在调节微生物定植中起关键作用，我们纵向研究了糖尿病伤口微生物组与宿主组织基因表达的串联。通过对16S核糖体RNA基因进行测序，我们发现伤口微生物群的纵向选择性转移与糖尿病小鼠的愈合受损相吻合(Lepr(db/db)；db / db)。我们证明了纵向基因表达的平行转移，发生在一组与免疫反应相关的基因中。此外，我们还建立了葡萄球菌相对丰度与皮肤防御反应基因表达之间的相关性。我们的数据表明，整合两种类型的全球数据集有助于更好地理解宿主-微生物相互作用的动力学。
Diabetics frequently suffer from chronic, nonhealing wounds. Although bacterial colonization and/or infection are generally acknowledged to negatively impact wound healing, the precise relationship between the microbial community and impaired wound healing remains unclear. Because the host cutaneous defense response is proposed to play a key role in modulating microbial colonization, we longitudinally examined the diabetic wound microbiome in tandem with host tissue gene expression. By sequencing 16S ribosomal RNA genes, we show that a longitudinal selective shift in wound microbiota coincides with impaired healing in diabetic mice (Lepr(db/db); db/db). We demonstrate a parallel shift in longitudinal gene expression that occurs in a cluster of genes related to the immune response. Further, we establish a correlation between relative abundance of Staphylococcus spp. and the expression of cutaneous defense response genes. Our data demonstrate that integrating two types of global datasets lends a better under standing to the dynamics governing host-microbe interactions.