A fluorescently labelled sialic acid for high performance intraoperative tumor detection
A fluorescently labelled sialic acid for high performance intraoperative tumor detection
复制标题
用于高性能术中肿瘤检测的荧光标记唾液酸
DOI：
10.1039/c4bm00028e
复制
发表时间：
2014-01-01
影响因子：
6.6
通讯作者：
Han, Shoufa
中科院分区：
文献类型：
--
作者：
Wu, Xuanjun;Tian, Yunpeng;Han, Shoufa
手术切除广泛用于肿瘤治疗，需要精确定位难以捉摸的肿瘤病灶的方法。本文报道了用异硫氰酸荧光素（FITC）标记的唾液酸（FITC-SA）对小鼠肿瘤的高效检测。对静脉注射FITC-SA的小鼠的分析显示，在皮下肿瘤和直径为0.2-5 mm的肝肿瘤植入物中，靶标与背景荧光比率较高，显著低于最小残留癌症的临床阈值（类似于1 cm清除率）。细胞外FITC-SA从循环中快速清除，而细胞内FITC-SA可以通过细胞唾液酸化途径代谢掺入糖蛋白中。与负载FITC-SA的纳米颗粒相比，游离FITC-SA优先且快速地被小鼠中的肿瘤摄取，并且显示出高的肿瘤与背景信号对比度，这表明了荧光定向手术消融肿瘤的潜力。
Surgical resection is widely used for tumor treatment, necessitating approaches for the precise locating of elusive tumor foci. We report the high performance detection of tumors in mice with fluorescein-isothiocyanate (FITC) labelled sialic acid (FITC-SA), a fluorescent monosaccharide with low cytoxicity. Analysis of mice intravenously injected with FITC-SA revealed high target-to-background fluorescence ratios in subcutaneous tumors and liver tumor implants with 0.2-5 mm diameters, which are significantly below the clinical threshold of minimal residual cancer (similar to 1 cm clearance). Extracellular FITC-SA is quickly cleared from circulation whereas the intracellular FITC-SA could be metabolically incorporated into glycoproteins via a cellular sialylation pathway. Compared with FITC-SA-laden nanoparticles, free FITC-SA is preferentially and quickly taken up by tumors in mice and displays high tumor-to-background signal contrast, suggesting the potential for fluorescence directed surgical ablation of tumors.