NUCLEOTIDE-SEQUENCE OF MOUSE IL-2 RECEPTOR CDNA AND ITS COMPARISON WITH THE HUMAN IL-2-RECEPTOR SEQUENCE
NUCLEOTIDE-SEQUENCE OF MOUSE IL-2 RECEPTOR CDNA AND ITS COMPARISON WITH THE HUMAN IL-2-RECEPTOR SEQUENCE
复制标题
DOI：
10.1093/nar/13.5.1505
复制
发表时间：
1985-01-01
影响因子：
14.9
通讯作者：
HONJO, T
中科院分区：
文献类型：
--
作者：
SHIMUZU, A;KONDO, S;HONJO, T
以人白细胞介素2受体cDNA为探针，从小鼠T细胞系CTLL中克隆了编码小鼠白细胞介素2受体的cDNA。用cDNA转染的COS 7细胞表达抗鼠IL-2受体的单克隆抗体识别的抗原。该cDNA在CTLL细胞中鉴定出4种小鼠IL-2受体的mRNA（4.5、3.5、2.2和1.5kb）。mRNA长度的差异似乎归因于3′非翻译序列的可变长度。测定了该cDNA的总核苷酸序列（约1400 bp），并与人受体进行了比较。IL-2受体的核苷酸和氨基酸序列在两个物种之间的平均同源性分别为70%和60%。比较发现了几个保守的区域定位于特定的外显子，如跨膜和细胞质部分，这表明这些区域是重要的受体功能和调节。
We have cloned cDNA encoding the mouse interleukin-2(IL-2) receptor from a murine T cell line, CTLL using human IL-2 receptor cDNA as probe. COS 7 cells transfected with the cDNA expressed the antigen recognized by the monoclonal antibody against the murine IL-2 receptor. The cDNA identified 4 species of mRNA (4.5, 3.5, 2.2 and 1.5 kb) of the mouse IL-2 receptor in CTLL cells. Difference in the length of mRNA seems to be ascribed to the variable length of the 3′ untranslated sequence. Total nucleotide sequence (˜1400 bp) of this cDNA was determined and compared with the human receptor. The nucleotide and amino acid sequences of the IL-2 receptor are 70% and 60%, respectively, homologous in average between the two species. The comparison has revealed several conserved regions localized to particular exons such as transmembrane and cytoplasmic portions, suggesting that these regions are important for receptor function and its regulation.