Rat ovarian angiotensin II receptors, renin, and angiotensin I-converting enzyme during pregnancy and the postpartum period.
Rat ovarian angiotensin II receptors, renin, and angiotensin I-converting enzyme during pregnancy and the postpartum period.
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妊娠和产后期大鼠卵巢血管紧张素 II 受体、肾素和血管紧张素 I 转换酶。
DOI：
10.1095/biolreprod47.6.925
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发表时间：
1992
影响因子：
3.6
通讯作者：
Husain,A
中科院分区：
文献类型：
--
作者：
Howard,RB;Husain,A
卵巢肾素-血管紧张素系统（RAS）已被广泛研究的处女骑自行车大鼠，但很少有关于这个系统在怀孕和产后大鼠的信息。我们发现，肾素和血管紧张素I转换酶（ACE）-参与血管紧张素II（Ang II）形成的关键酶-和Ang II受体，存在于怀孕和产后大鼠卵巢。从妊娠第2-4天至第10-12天，活性卵巢肾素的范围为1.12 ± 0.13至1.27 ± 0.19 ng Ang I/h/mg，占总（活性+非活性）卵巢肾素活性的68%至86%。在妊娠第10-12天和第14-16天，卵巢活性肾素活性略有增加，但非活性肾素消失，表明其活化;剩余的活性肾素在第18-20天下降62%（p< 0.05）。在产后第2天，活性和总卵巢肾素活性均超过妊娠第2-20天（p< 0.05）;产后第3天，两者水平均急剧下降（p< 0.05）。在妊娠大鼠中，通过[125 I]-[Sar 1，Ile 8]Ang II与卵巢膜的特异性结合鉴定的卵巢Ang II受体水平在妊娠第2-4天和第10-12天之间较高，范围为12.8 ± 1.7至15.7 ± 3.4 fmol/mg，但在妊娠第10-12天和第18-20天稳定下降82%（p< 0.05）。产后第2、3和4天的Ang II受体水平显示从与妊娠第18-20天相当的低水平逐渐增加。卵巢ACE活性在整个妊娠期或产后期间没有变化。血浆活性和总肾素活性往往是在怀孕期间高于产后第3和第4天。妊娠和产后大鼠卵巢中活性肾素、ACE和Ang II受体的存在，以及活性肾素和Ang II受体的变化，表明Ang II可调节妊娠和产后大鼠的卵巢功能。
The ovarian renin-angiotensin system (RAS) has been studied extensively in the virgin cycling rat, but little information is available about this system in pregnant and postpartum rats. We show that renin and angiotensin I-converting enzyme (ACE)—the key enzymes involved in angiotensin II (Ang II) formation—and Ang II receptors, are present in pregnant and postpartum rat ovaries. From gestation Days 2–4 to 10–12, active ovarian renin ranged from 1.12 ± 0.13 to 1.27 ± 0.19 ng Ang I/h/mg and comprised between 68 and 86% of total (active+inactive) ovarian renin activity. Between Days 10–12 and Days 14–16 of pregnancy, ovarian active renin activity increased slightly, but inactive renin disappeared, suggesting its activation; the remaining active renin then decreased 62% by Days 18–20 (p< 0.05). On postpartum Day 2, both active and total ovarian renin activity exceeded that of Days 2–20 of pregnancy (p< 0.05); levels of both then declined sharply by postpartum Day 3 (p< 0.05). In pregnant rats, levels of ovarian Ang II receptors, identified by the specific binding of [125I]-[Sar1,Ile8]Ang II to ovarian membranes, were high between Days 2–4 and 10–12 of pregnancy, ranging from 12.8 ± 1.7 to 15.7 ± 3.4 fmol/mg, but steadily declined by 82% between gestation Days 10–12 and 18–20 (p< 0.05). Postpartum Ang II receptor levels on Days 2, 3, and 4 showed a gradual increase from low levels comparable to Days 18–20 of pregnancy. Ovarian ACE activity did not change throughout pregnancy or during the postpartum period. Plasma active and total renin activity tended to be higher during pregnancy than during postpartum Days 3 and 4. The presence in pregnant and postpartum rat ovaries of active renin, ACE, and Ang II receptors, as well as changes in active renin and Ang II receptors, suggest that Ang II may regulate ovarian function in pregnant and postpartum rats.