Enzootic transmission of deer tick virus in New England and Wisconsin sites
Enzootic transmission of deer tick virus in New England and Wisconsin sites
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DOI：
10.4269/ajtmh.2000.63.36
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发表时间：
2000-07-01
影响因子：
3.3
通讯作者：
Telford, SR
中科院分区：
文献类型：
--
作者：
Ebel, GD;Campbell, EN;Telford, SR
为了确定是否啮齿动物强烈暴露于鹿蜱传播的莱姆病，人粒细胞埃立克体病，和人类巴贝西虫病的代理人也暴露于鹿蜱病毒（DTV），我们分析了白足小鼠（白足鼠）和草甸田鼠（田鼠pennsylvicus）在鹿蜱密集出没的地点的血清样本。为了进行血清学调查，我们通过克隆、表达和纯化DTV包膜糖蛋白（DTV rE）的一部分作为测试抗原，开发了酶联免疫吸附试验（ELISA）和Western印迹试验。通过ELISA筛选在马萨诸塞州、罗得岛和威斯康星州捕获的小鼠和田鼠的血清中对DTV rE具有反应性的IgG。随后通过免疫印迹法分析通过ELISA检测为阳性或临界的样品。认为两种试验中均具有反应性的样本为阳性。从罗得岛采集的264份小鼠样本中，3%呈阳性;从威斯康星州捕获的52份小鼠样本中，3.8%呈阳性;从马萨诸塞州南图克特岛捕获的282份小鼠样本中，3.9%呈阳性。来自马萨诸塞州大岛或任何研究中心的田鼠样本均无反应性。逆转录-聚合酶链反应产生的DTV感染探索成年鹿蜱的血清反应性小鼠被困的网站的分子证据，但不是从蜱收集的病毒永存的血清学证据是不存在的。白足鼠似乎暴露于DTV在某些网站，其他鹿蜱传播的代理人永久。这种病毒可以维持在同一个地方性流行周期。
To determine whether rodents that are intensely exposed to the deer tick-transmitted agents of Lyme disease, human granulocytic ehrlichiosis, and human babesiosis are also exposed to deer tick virus (DTV), we assayed serum samples from white-footed mice (Peromyscus leucopus) and meadow voles (Microtus pennsylvanicus) in sites densely infested by deer ticks. To conduct serosurveys, we developed an enzyme-linked immunosorbent assay (ELISA) and Western blot assay by cloning, expressing, and purifying a portion of the DTV envelope glycoprotein (DTV rE) for use as test antigen. Sera from mice and voles trapped in Massachusetts, Rhode Island, and Wisconsin were screened by ELISA for IgG reactive to DTV rE. Samples that were positive or borderline by ELISA were subsequently analyzed by immunoblotting. Samples reactive in both assays were considered to be positive. Three percent of 264 mouse samples collected from sites in Rhode Island, 3.8% of 52 samples from mice trapped in Wisconsin, and 3.9% of 282 samples collected from mice trapped on Nantucket Island, MA were positive. No samples from either Great Island, MA: or voles from any study site were reactive. A reverse transcriptase-polymerase chain reaction yielded molecular evidence of DTV infecting questing adult deer ticks in sites where seroreactive mice were trapped, but not from ticks collected where serologic evidence of virus perpetuation was absent. White-footed mice appear to be exposed to DTV in certain sites where other deer tick-borne agents perpetuate. This virus may be maintained in the same enzootic cycle.