Variation in rod and cone density from the fovea to the mid-periphery in healthy human retinas using adaptive optics scanning laser ophthalmoscopy
Variation in rod and cone density from the fovea to the mid-periphery in healthy human retinas using adaptive optics scanning laser ophthalmoscopy
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DOI：
10.1038/eye.2016.107
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发表时间：
2016-08-01
期刊：
影响因子：
3.9
通讯作者：
Doble, N.
中科院分区：
文献类型：
--
作者：
Wells-Gray, E. M.;Choi, S. S.;Doble, N.
目的应用自适应光学扫描激光眼底镜(AO-SLO)对健康人眼视网膜中央60°范围内视杆视锥感光细胞的镶嵌进行研究。方法5例受试者在680 nm波长处采集30°鼻侧视网膜(NR)至30°颞侧视网膜(TR)的视杆、视锥感受器图像。使用配准的平均图像来测量60×60微米感兴趣区域内的杆状和锥状密度和间距。结果中心凹附近平均峰值视锥密度为164 000+/-24 000个/mm(2)，30度正视和30度正视时分别降至6700+/-1500和5400+/-700个/mm(2)。视锥-视锥间距从中心凹的2.7+/-0.2微米增加到正反30度时的14.6+/-1.4微米和30度时的16.3+/-0.7微米。杆密度在25度NR(124 000+/-20 000棒/mm(2))和20度TR(120 000+/-12 000棒/mm(2))时达到峰值，偏心率越高，杆密度越低。鼻侧中心至中心杆间距最小，为25度(2.1+/-0.1微米)。在时间上，视杆间距在20度时最小(2.2+/-0.1微米)，然后在30度时增加到2.3+/-0.1微米。结论视杆和视锥密度与组织学和先前的AO-SLO研究结果一致。结果表明，成像能力的视网膜偏心率比以前报道的更高。这在最初影响外周视网膜的疾病中具有临床重要性，例如视网膜色素变性。
Purpose To characterize the rod and cone photoreceptor mosaic at retinal locations spanning the central 60 degrees in vivo using adaptive optics scanning laser ophthalmoscopy (AO-SLO) in healthy human eyes.Methods AO-SLO images (0.7 x 0.9 degrees) were acquired at 680 nm from 14 locations from 30 degrees nasal retina (NR) to 30 degrees temporal retina (TR) in 5 subjects. Registered averaged images were used to measure rod and cone density and spacing within 60 x 60 mu m regions of interest. Voronoi analysis was performed to examine packing geometry at all locations.Results Average peak cone density near the fovea was 164 000 +/- 24 000 cones/mm(2) and decreased to 6700 +/- 1500 and 5400 +/- 700 cones/mm(2) at 30 degrees NR and 30 degrees TR, respectively. Cone-to-cone spacing increased from 2.7 +/- 0.2 mu m at the fovea to 14.6 +/- 1.4 mu m at 30 degrees NR and 16.3 +/- 0.7 mu m at 30 degrees TR. Rod density peaked at 25 degrees NR (124 000 +/- 20 000 rods/mm(2)) and 20 degrees TR (120 000 +/- 12 000 rods/mm(2)) and decreased at higher eccentricities. Center-to-center rod spacing was lowest nasally at 25 degrees (2.1 +/- 0.1 mu m). Temporally, rod spacing was lowest at 20 degrees (2.2 +/- 0.1 mu m) before increasing to 2.3 +/- 0.1 mu m at 30 degrees TR.Conclusions Both rod and cone densities showed good agreement with histology and prior AO-SLO studies. The results demonstrate the ability to image at higher retinal eccentricities than reported previously. This has clinical importance in diseases that initially affect the peripheral retina such as retinitis pigmentosa.