Detection of an intermediate compartment involved in transport of alpha-factor from the plasma membrane to the vacuole in yeast.
Detection of an intermediate compartment involved in transport of alpha-factor from the plasma membrane to the vacuole in yeast.
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DOI：
10.1083/jcb.110.6.1911
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发表时间：
1990-06
期刊：
影响因子：
--
通讯作者：
Riezman H
中科院分区：
文献类型：
--
作者：
Singer B;Riezman H
α-因子是酿酒酵母的交配信息素之一，其特异性结合细胞质膜上的受体，被内化并递送到液泡，在液泡中被降解。在15摄氏度时，信息素的吸收率只受到轻微影响，而传递到液泡的速度明显减慢。一个运输中间体携带α-因子的液泡可以被可逆地捕获处理与代谢抑制剂，氮化钠和氟化钠。通过差速离心和密度梯度离心分析判断，该中间体与液泡和质膜不同。我们提出的证据表明，α-因子是保护蛋白酶消化的洗涤剂敏感的结构，这表明信息素驻留在一个囊泡隔室。我们认为这种中间体代表了一个内吞或前液泡区室，参与了从质膜到液泡的囊泡运输。
alpha-Factor, one of the mating pheromones of Saccharomyces cerevisiae, binds specifically to a receptor on the plasma membrane of a cells, is internalized and delivered to the vacuole, where it is degraded. At 15 degrees C the rate of pheromone uptake is only slightly affected while delivery to the vacuole is markedly slowed down. A transport intermediate carrying alpha-factor to the vacuole can be reversibly trapped by treatment with the metabolic inhibitors, NaN3 and NaF. This intermediate(s) is distinct from the vacuole and the plasma membrane as judged by differential and density gradient centrifugation analysis. We present evidence that the alpha-factor is protected from protease digestion by a detergent-sensitive structure, suggesting that the pheromone resides within a vesicular compartment. We propose that this intermediate(s) represents an endocytic or prevacuolar compartment(s) involved in vesicular traffic from the plasma membrane to the vacuole.