Genes related to the very early stage of ConA-induced fulminant hepatitis: a gene-chip-based study in a mouse model.
Genes related to the very early stage of ConA-induced fulminant hepatitis: a gene-chip-based study in a mouse model.
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与刀豆蛋白 A 诱导的暴发性肝炎极早期相关的基因：基于基因芯片的小鼠模型研究
DOI：
10.1186/1471-2164-11-240
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发表时间：
2010-04-15
期刊：
影响因子：
4.4
通讯作者：
Chen Z
中科院分区：
文献类型：
--
作者：
Chen F;Zhu HH;Zhou LF;Li J;Zhao LY;Wu SS;Wang J;Liu W;Chen Z
背景由于暴发性肝炎的发病率和死亡率较高，早期诊断、早期有效治疗是改善预后的关键。迄今为止，人们对这种严重疾病早期的基因表达变化知之甚少。鉴定与暴发性肝炎发生极早期相关的基因可为早期诊断提供精确线索。结果Balb/C小鼠注射ConA诱发暴发性肝炎，经病理生化检查证实。经过基于基因芯片的筛选，通过方差分析、基因表达谱、基因网络构建和实时RT-PCR进一步剖析肝脏中的基因表达数据。在ConA引发的暴发性肝炎的早期阶段，总共鉴定了1,473个具有不同表达变异的基因。其中，最终选择了26个基因进行进一步研究。基因网络分析数据表明MPDZ和Acsl1这两个基因定位于网络的核心。结论在暴发性肝炎的早期阶段,涉及蛋白质转运、转录调控和细胞代谢的26个基因的表达发生了显着改变。这些基因形成一个网络，并显示出与暴发性肝炎的发展密切相关。我们的研究为暴发性肝炎的早期诊断提供了几个潜在的目标。
BackgroundDue to the high morbidity and mortality of fulminant hepatitis, early diagnosis followed by early effective treatment is the key for prognosis improvement. So far, little is known about the gene expression changes in the early stage of this serious illness. Identification of the genes related to the very early stage of fulminant hepatitis development may provide precise clues for early diagnosis.ResultsBalb/C mice were used for ConA injection to induce fulminant hepatitis that was confirmed by pathological and biochemical examination. After a gene chip-based screening, the data of gene expression in the liver, was further dissected by ANOVA analysis, gene expression profiles, gene network construction and real-time RT-PCR.At the very early stage of ConA-triggered fulminant hepatitis, totally 1,473 genes with different expression variations were identified. Among these, 26 genes were finally selected for further investigation. The data from gene network analysis demonstrate that two genes, MPDZ and Acsl1, localized in the core of the network.ConclusionsAt the early stages of fulminant hepatitis, expression of twenty-six genes involved in protein transport, transcription regulation and cell metabolism altered significantly. These genes form a network and have shown strong correlation with fulminant hepatitis development. Our study provides several potential targets for the early diagnosis of fulminant hepatitis.
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影响因子：
15.3
作者：
Kaneko, Y;Harada, M;Kawano, T;Yamashita, M;Shibata, Y;Gejyo, F;Nakayama, T;Taniguchi, M
通讯作者：
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
Simon, RM
DOI：
10.1073/pnas.0403627101
发表时间：
2004-11-30
影响因子：
11.1
作者：
Gracey, AY;Fraser, EJ;Cossins, AR
通讯作者：
Cossins, AR