Antioxidant defense mechanism under salt stress in wheat seedlings
Antioxidant defense mechanism under salt stress in wheat seedlings
复制标题
DOI：
10.1007/s10535-006-0011-7
复制
发表时间：
2006-06-01
影响因子：
1.5
通讯作者：
Sawhney, V
中科院分区：
文献类型：
--
作者：
Mandhania, S;Madan, S;Sawhney, V
以耐盐小麦品种KRL-19和盐敏感品种WH-542为材料，研究了盐胁迫对小麦幼苗细胞膜损伤、离子含量和抗氧化酶的影响。用0、50和100 mM的氯化钠对4d的幼苗进行浇灌。分别在盐处理后第3天、第6天和除盐后第2天记录观察结果。相对含水量随盐胁迫的诱导而下降，WH-542的下降幅度大于Cv。KRL-19。K+/Na+比值KRL-19高于WH-542。与KRL-19相比，WH-542由于脂质过氧化对细胞膜的损伤更大，表现为更高的过氧化氢和丙二醛积累以及更大的电解质泄漏。两个品种的过氧化氢酶、过氧化物酶、抗坏血酸过氧化物酶和谷胱甘肽还原酶活性均随盐胁迫程度的增加而升高，而超氧化物歧化酶活性则下降。脱盐后，在KRL-19中观察到这些酶的活性部分恢复，而在WH-542中恢复非常缓慢。
The present study was carried out to study the effect of salt stress on cell membrane damage, ion content and antioxidant enzymes in wheat (Triticum aestivum) seedlings of two cultivars salt-tolerant KRL-19 and salt-sensitive WH-542. Seedlings (4-d-old) were irrigated with 0, 50 and 100 mM NaCl. Observations were recorded on the 3(rd) and 6(th) day after salt treatment and 2(nd) day after salt removal. The relative water content declined with induction of salt stress, more in WH-542 than in cv. KRL-19. K+/Na+ ratio in KRL-19 was higher than in WH-542. WH-542 suffered greater damage to cellular membranes due to lipid peroxidation as indicated by higher accumulation of H2O2, MDA and greater leakage of electrolytes than KRL-19. The activities of catalase, peroxidase and ascorbate peroxidase and glutathione reductase increased with increase in salt stress in both the cultivars, however, superoxide dismutase activity declined. Upon desalanization, partial recovery in the activities of these enzymes was observed in KRL-19 and very slow recovery in WH-542.