Lasting effects of glutamate on nuclear calcium concentration in cultured rat hippocampal neurons: Regulation by calcium stores
Lasting effects of glutamate on nuclear calcium concentration in cultured rat hippocampal neurons: Regulation by calcium stores
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DOI：
10.1113/jphysiol.1996.sp021663
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发表时间：
1996-10-01
影响因子：
5.5
通讯作者：
Segal, M
中科院分区：
文献类型：
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作者：
Korkotian, E;Segal, M
1.采用Fura-2和Fluo-3钙离子指示剂测定培养大鼠海马神经元胞核和核周区细胞内游离钙浓度（[Ca 2 +]（i））的变化.短暂应用谷氨酸引起所有细胞隔室中[Ca 2 +]（i）的短暂升高，除核区域外，所有细胞隔室均恢复至给药前水平。新的，更高水平的核钙（[Ca 2 +]（n））持续只要细胞被监测. [Ca 2 +]（n）的新水平依赖于钙瞬变的幅度，并且在培养的较老细胞中更高，但它不影响对随后应用谷氨酸的反应。影响钙储存的药物（咖啡因、兰尼碱和钌红）可阻止[Ca 2 +]（n）的持续升高，表明细胞核内的钙储存与[Ca 2 +]（n）相互作用。
1. Changes in free intracellular calcium concentrations ([Ca2+](i)) were measured in the nucleus and perinuclear regions of cultured rat hippocampal neurons using either fura-2 or fluo-3 calcium indicators.2. Brief application of glutamate caused a transient rise of [Ca2+](i) in all cell compartments, which recovered to pre-drug levels in all but the nuclear region. The new, higher level of nuclear calcium ([Ca2+](n)) was sustained for as long as the cell was monitored.3. The new level of [Ca2+](n) was dependent on the magnitude of the calcium transient, and was higher in older cells in culture, but it did not affect responses to subsequent applications of glutamate.4. The sustained elevation of [Ca2+](n) was prevented by drugs which affect calcium stores (caffeine, ryanodine and Ruthenium Red), indicating that an extranuclear calcium store interacts with [Ca2+](n).