Thromboxane synthase is preferentially conserved in activated mouse peritoneal macrophages.
Thromboxane synthase is preferentially conserved in activated mouse peritoneal macrophages.
复制标题
血栓烷合酶优先保守于活化的小鼠腹膜巨噬细胞中。
DOI：
10.1172/jci112051
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Needleman,P
中科院分区：
文献类型：
--
作者：
Tripp,CS;Leahy,KM;Needleman,P
从未感染动物分离的驻留巨噬细胞产生大量花生四烯酸（AA）代谢物。用蛋白质抗原或细菌免疫动物激活巨噬细胞，并导致环氧合酶和脂氧合酶代谢物相对于驻留细胞减少80%。由于一些产品已被证明可以调节免疫功能，我们研究了AA代谢酶活性如何调节合成的产品。我们证明，环氧合酶，5-脂氧合酶，前列环素合酶，并可能前列腺素（PG）内过氧化物E-异构酶的活动在活化的腹腔巨噬细胞减少。与此形成鲜明对比的是，在活化的巨噬细胞中，血栓烷合酶活性选择性地不变或增强。因此，免疫反应似乎调节AA和PG内过氧化物依赖性酶的活性，从而决定了巨噬细胞合成的代谢产物谱的重大转变。
Resident macrophages isolated from uninfected animals produce large quantities of arachidonic acid (AA) metabolites. Immunizing animals with protein antigens or bacteria activates macrophages and causes an 80% reduction in the cyclooxygenase and lipoxygenase metabolites relative to resident cells. Since some products have been shown to modulate immune functions, we examined how the AA metabolic enzyme activities regulate the products that are synthesized. We demonstrate that the cyclooxygenase, 5-lipoxygenase, prostacyclin synthase, and probably prostaglandin (PG) endoperoxide E-isomerase activities were decreased in activated peritoneal macrophages. In sharp contrast, thromboxane synthase activity was selectively unchanged or enhanced in the activated macrophages. Thus the immune response appears to modulate the activity of the AA and PG endoperoxide-dependent enzymes, thus dictating a major shift in the profile of metabolites synthesized by macrophages.