ELECTRON-SPIN ECHO STUDIES OF THE COPPER-BINDING SITE IN PHENYLALANINE-HYDROXYLASE FROM CHROMOBACTERIUM-VIOLACEUM
ELECTRON-SPIN ECHO STUDIES OF THE COPPER-BINDING SITE IN PHENYLALANINE-HYDROXYLASE FROM CHROMOBACTERIUM-VIOLACEUM
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DOI：
10.1021/ja00212a012
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发表时间：
1988-02-17
影响因子：
15
通讯作者：
PEISACH, J
中科院分区：
文献类型：
--
作者：
MCCRACKEN, J;PEMBER, S;PEISACH, J
利用电子自旋回波技术研究了紫色色杆菌（Chromobacterium violaceum）苯丙氨酸羟化酶的活性中心结构。傅立叶变换的受激电子自旋回波包络的铜蛋白揭示了铜组氨酰咪唑协调的频率成分的特点。在0.55，1.0，和1.55 MHz所观察到的核四极频率略有不同的模型铜（II）-咪唑配合物，0.68，0.72，和1.40 MHz，但可以观察到在铜（II）配合物与2-甲基咪唑。提出了一种光谱模拟分析方法，用于定量赤道咪唑配位Cu（II）的数量。在苯丙氨酸羟化酶中，两个是结合的。
The active-site structure of the Cu(II)-containing phenylalanine hydroxylase from Chromobacterium violaceum was studied by electron spin-echo spectroscopy. Fourier transformation of the stimulated electron spin-echo envelope for the copper protein revealed frequency components characteristics of copper-histidyl imidazole coordination. The observed nuclear quadrupole frequencies at 0.55, 1.0, and 1.55 MHz are slightly different from those observed for model Cu(II)-imidazole complexes, 0.68, 0.72, and 1.40 MHz, but can be observed in Cu(II) complexes with 2-methylimidazole. A method of analysis by spectral simulation is presented for the quantitation of the number of equatorial imidazoles coordinated to Cu (II). In phenylalanine hydroxylase, two are bound.