Ubiquitin C-terminal Hydrolase 37, a novel predictor for hepatocellular carcinoma recurrence, promotes cell migration and invasion via interacting and deubiquitinating PRP19
Ubiquitin C-terminal Hydrolase 37, a novel predictor for hepatocellular carcinoma recurrence, promotes cell migration and invasion via interacting and deubiquitinating PRP19
复制标题
泛素 C 末端水解酶 37 是肝细胞癌复发的新型预测因子，通过与 PRP19 相互作用和去泛素化来促进细胞迁移和侵袭。
DOI：
10.1016/j.bbamcr.2012.11.020
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发表时间：
2013-03-01
影响因子：
5.1
通讯作者：
Shen, Xizhong
中科院分区：
文献类型：
--
作者：
Fang, Ying;Fu, Da;Shen, Xizhong
泛素C末端水解酶37（UCH 37）在许多生物学过程中起着至关重要的作用，也参与肿瘤的发生。在这项研究中，临床病理数据显示，UCH 37在肝细胞癌（HCC）癌组织中过表达，是复发时间（TTR）的重要预测因子。在体外，我们发现UCH 37可以促进细胞的迁移和侵袭。随后，我们利用Isobaric Tags for Relative and Absolute Quantitation（iTRAQ）来鉴定UCH 37过表达细胞与对照细胞相比差异表达的蛋白质，并发现PRP19，一种必需的RNA剪接因子，被上调。进一步证实了UCH 37、PRP 19与细胞迁移和侵袭能力的关系。总的来说，这项研究表明，UCH 37可以通过相互作用和去泛素化PRP19来促进HCC细胞系中的细胞迁移和侵袭，并表明UCH 37可以成为HCC根治性切除术后复发的新预测因子。(C)2012爱思唯尔有限公司版权所有。
Ubiquitin C-terminal hydrolase 37 (UCH37) plays a crucial role in numerous biological processes and is also involved in oncogenesis. In this study, clinicopathologic data showed that UCH37 was over-expressed in hepatocellular carcinoma (HCC) cancerous tissues and was a significant predictor for time to recurrence (TTR). In vitro, we discovered that UCH37 could promote cell migration and invasion. Subsequently, we utilized Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) to identify differentially expressed proteins in UCH37 over-expressing cells compared with the control cells, and found that PRP19, an essential RNA splicing factor, was up-regulated. The relationship between UCH37, PRP19 and the capability of cell migration and invasion was further confirmed. Collectively, this study demonstrated that UCH37 could promote cell migration and invasion in HCC cell lines through interacting and deubiquitinating PRP19, and suggested that UCH37 could be a novel predictor for HCC recurrence after curative resection. (C) 2012 Elsevier B.V. All rights reserved.