A petal-specific InMYB1 promoter from Japanese morning glory: a useful tool for molecular breeding of floricultural crops
A petal-specific InMYB1 promoter from Japanese morning glory: a useful tool for molecular breeding of floricultural crops
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DOI：
10.1111/pbi.12389
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发表时间：
2016-01-01
影响因子：
13.8
通讯作者：
Shiratake, Katsuhiro
中科院分区：
文献类型：
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作者：
Azuma, Mirai;Morimoto, Reina;Shiratake, Katsuhiro
生产具有改变的花瓣特性的新型转基因花卉作物需要赋予有用性状的转基因和花瓣特异性启动子。几种启动子已经显示出控制花瓣中的转基因。然而，所有这些都具有固有的缺点，例如在开花阶段花瓣特异性低和活性受限。此外，没有检查启动子在广泛的植物物种中赋予花瓣特异性表达的能力。在这里，我们报告的InMYB 1的启动子从日本牵牛花作为一种新的花瓣特异性启动子的分子育种的花卉作物。首先，我们生产了稳定的InMYB1_1kb：：GUS转基因拟南芥和洋桔梗植物，并通过组织化学β-葡萄糖醛酸酶（GUS）染色表征了InMYB 1启动子控制下的空间和时间表达模式。GUS染色模式仅在花瓣中观察到。该结果表明，InMYB 1启动子作为花瓣特异性启动子发挥功能。其次，我们通过基因枪法将InMYB 1_1kb：：GUS基因瞬时导入到洋桔梗、菊花、康乃馨、龙胆草、砧木、玫瑰、石斛和百合花瓣中。在洋桔梗、菊花、康乃馨、龙胆草和砧木中观察到GUS染色斑点。这些结果表明InMYB 1启动子在大多数双子叶植物中起作用。第三，为了显示InMYB 1启动子在分子育种中的实用性，在拟南芥中，在InMYB 1启动子的控制下抑制或增强MIXTA样基因的功能。转基因植株仅表现出明显的形态学变化，即花瓣起皱。这些结果表明，InMYB 1启动子可作为花瓣特异性启动子应用于花卉作物的分子育种。
Production of novel transgenic floricultural crops with altered petal properties requires transgenes that confer a useful trait and petal-specific promoters. Several promoters have been shown to control transgenes in petals. However, all suffer from inherent drawbacks such as low petal specificity and restricted activity during the flowering stage. In addition, the promoters were not examined for their ability to confer petal-specific expression in a wide range of plant species. Here, we report the promoter of InMYB1 from Japanese morning glory as a novel petal-specific promoter for molecular breeding of floricultural crops. First, we produced stable InMYB1_1kb::GUS transgenic Arabidopsis and Eustoma plants and characterized spatial and temporal expression patterns under the control of the InMYB1 promoter by histochemical b-glucuronidase (GUS) staining. GUS staining patterns were observed only in petals. This result showed that the InMYB1 promoter functions as a petal-specific promoter. Second, we transiently introduced the InMYB1_1kb::GUS construct into Eustoma, chrysanthemum, carnation, Japanese gentian, stock, rose, dendrobium and lily petals by particle bombardment. GUS staining spots were observed in Eustoma, chrysanthemum, carnation, Japanese gentian and stock. These results showed that the InMYB1 promoter functions in most dicots. Third, to show the InMYB1 promoter utility in molecular breeding, a MIXTA-like gene function was suppressed or enhanced under the control of InMYB1 promoter in Arabidopsis. The transgenic plant showed a conspicuous morphological change only in the form of wrinkled petals. Based on these results, the InMYB1 promoter can be used as a petal-specific promoter in molecular breeding of floricultural crops.