The beneficial effect of melatonin in brain endothelial cells against oxygen-glucose deprivation followed by reperfusion-induced injury.
The beneficial effect of melatonin in brain endothelial cells against oxygen-glucose deprivation followed by reperfusion-induced injury.
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DOI：
10.1155/2014/639531
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发表时间：
2014
影响因子：
--
通讯作者：
Lee JE
中科院分区：
文献类型：
--
作者：
Song J;Kang SM;Lee WT;Park KA;Lee KM;Lee JE
褪黑激素对脑血管和神经退行性疾病具有细胞保护作用。保护脑内皮细胞免受缺氧和氧化应激对于治疗中枢神经系统（CNS）疾病非常重要，因为脑内皮细胞构成血脑屏障（BBB）。在本研究中，我们研究了褪黑激素对 bEnd.3 细胞中氧糖剥夺以及再灌注 (OGD/R-) 诱导的损伤的保护作用。通过蛋白质印迹分析、细胞活力测定、细胞内活性氧 (ROS) 测量和免疫细胞化学 (ICC) 来检查褪黑激素的作用。我们的结果表明，在 OGD/R 诱导的损伤中，褪黑激素治疗可以防止细胞死亡和紧密连接蛋白的降解。为了响应 bEnd.3 细胞的 OGD/R 损伤，褪黑素激活 Akt，从而促进细胞存活，并减弱 JNK 的磷酸化，从而引发细胞凋亡。因此，褪黑激素可以保护 bEnd.3 细胞免受 OGD/R 诱导的损伤。
Melatonin has a cellular protective effect in cerebrovascular and neurodegenerative diseases. Protection of brain endothelial cells against hypoxia and oxidative stress is important for treatment of central nervous system (CNS) diseases, since brain endothelial cells constitute the blood brain barrier (BBB). In the present study, we investigated the protective effect of melatonin against oxygen-glucose deprivation, followed by reperfusion- (OGD/R-) induced injury, in bEnd.3 cells. The effect of melatonin was examined by western blot analysis, cell viability assays, measurement of intracellular reactive oxygen species (ROS), and immunocytochemistry (ICC). Our results showed that treatment with melatonin prevents cell death and degradation of tight junction protein in the setting of OGD/R-induced injury. In response to OGD/R injury of bEnd.3 cells, melatonin activates Akt, which promotes cell survival, and attenuates phosphorylation of JNK, which triggers apoptosis. Thus, melatonin protects bEnd.3 cells against OGD/R-induced injury.
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DOI：
10.1097/01.wcb.0000091762.61714.fe
发表时间：
2003-11-01
影响因子：
6.3
作者：
Andjelkovic, AV;Stamatovic, SM;Keep, RF
通讯作者：
Keep, RF
影响因子：
2.9
作者：
Abbruscato, TJ;Davis, TP
通讯作者：
Davis, TP
影响因子：
10.3
作者：
Dai, Min;Cui, Peilin;Xiu, Ruijuan
通讯作者：
Xiu, Ruijuan
同型半胱氨酸激活JNK信号引起骨髓间充质干细胞凋亡
影响因子：
3.7
作者：
Cai B;Li X;Wang Y;Liu Y;Yang F;Chen H;Yin K;Tan X;Zhu J;Pan Z;Wang B;Lu Y
通讯作者：
Lu Y
影响因子：
10.3
作者：
Ersahin, Mehmet;Toklu, Hale Z.;Sener, Goksel
通讯作者：
Sener, Goksel