Lectin binding patterns and immunohistochemical antigen detection in placenta and lungs of Brucella abortus-bovine infected fetuses.
Lectin binding patterns and immunohistochemical antigen detection in placenta and lungs of Brucella abortus-bovine infected fetuses.
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DOI：
10.4314/ovj.v8i1.10
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发表时间：
2018
影响因子：
1.2
通讯作者：
Barbeito CG
中科院分区：
文献类型：
--
作者：
Fiorentino MA;Paolicchi FA;Campero CM;Barbeito CG
凝集素结合依赖于这些物质对特定末端糖的亲和力。该方法有助于鉴定末端糖所连接的复杂结构，并可揭示细胞、细胞内相互作用或细胞外转运途径中的生理或病理变化。本研究旨在研究牛种布氏杆菌感染对牛胎肺（n=6）和牛胎盘（n=5）凝集素结合模式的影响。用B进行实验性接种的小母牛的胎肺和胎盘。用组织学、凝集素组织化学、免疫组织化学和培养技术对2308株牛流产杆菌进行了观察。B。免疫组化法检测胎肺和胎盘中的流产抗原。UEA-1、DBA、PNA、RCA-1和SBA在肺中的标记增加，UEA-1、ConA、PNA、DBA在胎盘中的标记增加。本凝集素组织化学研究揭示了一个独特的模式寡糖分布在肺和胎盘的B。流产感染的胎儿
Lectin binding relies on the affinity of these substances for specific terminal sugars. The method facilitates the identification of complex structures to which the terminal sugar attaches and may reveal physiological or pathological changes in cells, intracellular interactions or extracellular transport pathways. This study was carried out to investigate the effect of infection with Brucella abortus on the pattern of lectin binding in bovine fetal lungs (n=6) and bovine placentas (n=5). Fetal lungs and placenta from heifers experimentally inoculated with B. abortus, strain 2308 were examined by histological, lectin-histochemical, immunohistochemical and cultural techniques. B. abortus antigens were immunohistochemically detected in fetal lungs and placenta. An increase in the labeling with UEA-1, DBA, PNA, RCA-1 and SBA was found in the lungs and an increase in the labeling with UEA-1, ConA, PNA, DBA was found in the placentas. The present lectin histochemical study revealed a distinctive pattern of oligosaccharide distribution in the lungs and placenta of B. abortus-infected fetuses.