Characteristic Oxidation Products of Choline Plasmalogens are Detectable in Cattle and Roe Deer Spermatozoa by MALDI-TOF Mass Spectrometry
Characteristic Oxidation Products of Choline Plasmalogens are Detectable in Cattle and Roe Deer Spermatozoa by MALDI-TOF Mass Spectrometry
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通过 MALDI-TOF 质谱法可在牛和狍子精子中检测到胆碱缩醛磷脂的特征氧化产物
DOI：
10.1007/s11745-007-3108-7
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发表时间：
2007
期刊：
影响因子：
1.9
通讯作者：
J. Schiller
中科院分区：
文献类型：
--
作者：
B. Fuchs;K. Müller;F. Göritz;S. Blottner;J. Schiller
缩醛磷脂（1-O-alk-1′-enyl-2-acyl-sn-glycero-3-phosphocholines和-phosphoethanolamines）是精子膜的重要组成部分，具有重要的抗氧化作用。这尤其适用于来自精子的缩醛磷脂也具有非常高含量的高度不饱和脂肪酰基残基（特别是22：6）。采用基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱法分析了两种反刍动物（牛和狍）精子有机提取物中特征性胆碱酶原氧化产物的含量。结果表明，1-羟基-2-二十二碳六烯酰-sn-甘油-3-磷酸胆碱（LPC 22：6）和甲酰-LPC 22：6是精子脂质氧化的可靠指标，因此可以得出有关储存条件的结论。通过研究选定参比化合物的氧化行为，也证实了精子的所有数据。它将被证明，同样，如果使用缩醛磷脂或二酰基PC物种，氧化主要发生在双键旁边的甘油骨架。通过记录相应的源后衰变（PSD）碎片离子谱，进一步证实了这些数据。
Plasmalogens (1-O-alk-1′-enyl-2-acyl-sn-glycero-3-phosphocholines and -phosphoethanolamines) are important constituents of spermatozoa membranes and possess significant antioxidative properties. This particularly holds as plasmalogens from spermatozoa also possess a very high content of highly unsaturated fatty acyl residues (especially 22:6). The organic spermatozoa extracts of two different ruminants (cattle and roe deer) were analyzed for their contents of characteristic choline plasmalogen oxidation products by matrix-assisted laser desorption and ionization time-of-flight (MALDI-TOF) mass spectrometry. It will be shown that 1-hydroxy-2-docosahexaenoyl-sn-glycero-3-phosphocholine (LPC 22:6) and formyl-LPC 22:6 are reliable measures of lipid oxidation of spermatozoa and allow, accordingly, conclusions about the storage conditions. All data on spermatozoa were also confirmed by the investigation of the oxidation behavior of selected reference compounds. It will be shown that, equally if plasmalogens or diacyl PC species are used, oxidation takes place primarily at the double bond next to the glycerol backbone. These data were additionally confirmed by recording the corresponding post source decay (PSD) fragment ion spectra.
影响因子：
7.4
作者：
Khaselev, N;Murphy, RC
通讯作者：
Murphy, RC