Sex chromosomes and sex-specific molecular markers in Indo-Pacific combtooth blennies (Blenniidae, Istiblennius)
Sex chromosomes and sex-specific molecular markers in Indo-Pacific combtooth blennies (Blenniidae, Istiblennius)
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DOI：
10.3354/meps13082
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发表时间：
2019-09-26
影响因子：
2.5
通讯作者：
Gamble, Tony
中科院分区：
文献类型：
--
作者：
Hundt, Peter J.;Liddle, Emma B.;Gamble, Tony
性别特异性遗传标记是在一种性别中发现的标记，但在另一种性别中没有发现，在传统核型分析失败的情况下，可以用来识别物种的性染色体系统。具有雄性特异标记的物种具有XX/XY系统，而具有雌性特有标记的物种具有ZZ/ZW系统。在这里，我们使用了来自两种梳齿鱼Istiblennius lineatus和I.steindachneri的限制性酶切位点相关DNA测序(RADseq)的数据来识别过量的雌性特异遗传标记，这些标记指向ZZ/ZW性染色体。我们使用聚合酶链式反应来验证其中一个雌性特异性限制位点相关的DNA标记在另一个Istiblennius物种，I.edentulus的性别特异性。在其他2种Istiblennius和2种Blenniella中没有观察到性别特异性的PCR扩增。这个Istiblennius ZZ/ZW系统，当与文献中描述Parablennius的XX/XY系统的细胞遗传学数据相结合时，建立了斑潜蝇科性染色体系统之间的转换。
Sex-specific genetic markers, markers found in one sex but not the other, can be used to recognize a species' sex chromosome system in cases where traditional karyotyping fails. Species with male-specific markers have an XX/XY system, while species with female-specific markers have a ZZ/ZW system. Here, we used data from restriction site-associated DNA sequencing, or RADseq, from 2 species of combtooth blenny, Istiblennius lineatus and I. steindachneri, to identify an excess of female-specific genetic markers, which points to ZZ/ZW sex chromosomes. We used PCR to validate the sex-specificity of one of these female-specific restriction site-associated DNA markers in an additional Istiblennius species, I. edentulus. We observed no sex-specific PCR amplification in 2 other Istiblennius species and 2 Blenniella species. This Istiblennius ZZ/ZW system, when combined with cytogenetic data from the literature illustrating an XX/XY system in Parablennius, establishes a transition between sex chromosome systems within Blenniidae.