Ultradian components in the locomotor activity rhythms of the genetically normal mouse, Mus musculus
Ultradian components in the locomotor activity rhythms of the genetically normal mouse, Mus musculus
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DOI：
10.1242/jeb.038877
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发表时间：
2010-05-15
影响因子：
2.8
通讯作者：
Koide, Tsuyoshi
中科院分区：
文献类型：
--
作者：
Dowse, Harold;Umemori, Juzoh;Koide, Tsuyoshi
据报道，多种生物体的生理过程具有超电周期性，并且可能表现为运动活动的发作。在某些情况下，这种时间组织可能与某些行为学策略有关。然而，在小鼠中，据报道，超昼夜节律主要发生在因遗传或手术操作而扰乱昼夜节律起搏器的动物中。使用能够在存在强昼夜周期性的情况下解析超电范围周期性的分析技术，我们发现了小鼠中受光：暗循环影响的超电节律的明确证据。我们收集了 11 种基因不同的小鼠品系的个体的运动活动数据，这些小鼠的活动在 12 小时：12 小时 L：D 光周期下记录了 3 天。在过滤去除 24 小时周期性之前和之后，对数据进行最大熵谱分析和自相关。我们发现每个品系都有大多数个体具有强的超电节律，范围从大约 3 小时到大约 5 小时。这些周期性在个体动物中在高通滤波和未滤波的数据中通常是可见的。此外，当汇集给定菌株的所有原始数据以获得所有动物和天数的 24 小时整体平均值时，节律仍然是可辨别的。我们将傅里叶级数拟合到这些形式估计中，以模拟每个应变的频率结构，并发现应变的显着影响以及周期和应变之间的相互作用，表明超电范围内节律性的显着遗传变异。这项研究中使用的技术应该在一系列生物体和领域有更广泛的用途。
Ultradian periodicities in physiological processes have been reported for a wide variety of organisms and may appear as bouts in locomotor activity. In some instances, this temporal organization can be related to some ethological strategy. In mice, however, ultradian rhythms have been reported largely in animals with circadian pacemakers disrupted either by genetic or surgical manipulation. Using analysis techniques capable of resolving periodicities in the ultradian range in the presence of strong diel periodicity, we found unequivocal evidence of ultradian rhythms in mice entrained to an light: dark cycle. We collected locomotor activity data of individuals from 11 genetically disparate strains of mice whose activity was recorded in 12 h:12 h L:D photoperiods for 3 days. Data were subjected to maximum entropy spectral analysis and autocorrelation, both before and after filtering to remove the 24-h periodicity. We found that every strain had a majority of individuals with strong ultradian rhythms ranging from similar to 3 to similar to 5 h. These periodicities were commonly visible in individual animals both in high-pass-filtered and in unfiltered data. Furthermore, when all raw data from a given strain were pooled to get a 24-h ensemble average across all animals and days, the rhythms continued to be discernable. We fitted Fourier series to these form estimates to model the frequency structure of each strain and found significant effects of strain and an interaction between period and strain indicating significant genetic variation for rhythmicity in the ultradian range. The techniques employed in this study should have wider use in a range of organisms and fields.