Radioprotective effects of (-)-epigallocatechin-3-gallate on human erythrocyte/granulocyte lineages.
Radioprotective effects of (-)-epigallocatechin-3-gallate on human erythrocyte/granulocyte lineages.
复制标题
(-)-表没食子儿茶素-3-没食子酸酯对人红细胞/粒细胞谱系的辐射防护作用。
DOI：
10.1093/rpd/ncs226
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发表时间：
2012
影响因子：
1
通讯作者：
Kashiwakura I.
中科院分区：
文献类型：
--
作者：
Monzen S;Kashiwakura I.
表没食子儿茶素-3-没食子酸酯（EGCg）被广泛认为是一种强大的抗氧化剂和自由基清除剂。本研究检测了EGCg对人粒系和红系造血的辐射防护作用。从人胎盘/脐带血制备高度纯化的人CD 34+造血干/祖细胞。将细胞暴露于X射线，剂量率为101戈伊min-1，然后在添加粒细胞集落刺激因子（G-CSF）或促红细胞生成素（EPO）的培养基中培养。在X射线照射之前或之后立即将EGCg（100 nM）添加到培养物中。在作者先前的研究中确定了100 nM EGCg的浓度。经X射线照射的CD 34+细胞产生的粒细胞和红细胞集落数量呈剂量依赖性下降。虽然EGCg的加入使每种造血祖细胞的增殖增加了10.2倍，但在粒细胞祖细胞的存活分数中没有观察到显著的保护作用（单独的G-CSF：D 0 =1.06戈伊，n=1.14）。然而，与对照组相比，在照射前或照射后添加EGCg对红细胞集落形成具有显著更高的保护作用（单独EPO：D 0 =0.66戈伊，n=1.56; EGCg（照射前）：D 0 =0.43戈伊，n=5.48）。照射前加入EGCg可显著提高<1戈伊照射后红系祖细胞的存活率。这些结果表明，EGCg对辐射损伤的红系造血比粒系造血更有保护作用。
Epigallocatechin-3-gallate (EGCg) is widely recognised as a powerful antioxidant and free radical scavenger. This study examined the radioprotective effects of EGCg on human granulopoiesis and erythropoiesis. Highly purified human CD34+haematopoietic stem/progenitor cells were prepared from human placental/umbilical cord blood. The cells were exposed to X rays at a dose rate of ∼1 Gy min−1and then cultured in a medium supplemented with either granulocyte colony-stimulating factor (G-CSF) or erythropoietin (EPO). EGCg (100 nM) was added to the culture immediately before or after X-irradiation. The concentration of 100-nM EGCg was determined in the authors’ previous study. The number of granulocyte and erythrocyte colonies generated by X-irradiated CD34+cells decreased in a dose-dependent manner. Although EGCg addition yielded an ∼2-fold increase in the proliferation of each haematopoietic progenitor, no significant protective effect was observed in the surviving fraction of granulocyte progenitors (G-CSF alone:D0=1.06 Gy,n=1.14). However, EGCg addition before or after irradiation conferred a significantly higher protective effect on erythrocyte colony formation compared with the control (EPO alone:D0=0.66 Gy,n=1.56; EGCg (before):D0=0.43 Gy,n=5.48). EGCg addition before irradiation significantly improved the survival of erythroid progenitors subjected to radiation of <1 Gy. These results suggest that EGCg is more protective of erythropoiesis than granulopoiesis from radiation damage.