UPLC-HRMS-Based Plasma Metabolomic Profiling of Novel Biomarkers by Treatment with KDZI in Cerebral Ischemia Reperfusion Rats.
UPLC-HRMS-Based Plasma Metabolomic Profiling of Novel Biomarkers by Treatment with KDZI in Cerebral Ischemia Reperfusion Rats.
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基于 UPLC-HRMS 的脑缺血再灌注大鼠中 KDZI 治疗新型生物标志物的血浆代谢组学分析
DOI：
10.3390/molecules23061315
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发表时间：
2018-05-30
期刊：
影响因子：
--
通讯作者：
Zhao B
中科院分区：
文献类型：
--
作者：
Wang C;Liu C;Wang M;Ma Q;Li Y;Wang T;Zhao B
苦碟子注射液（KDZI），又称碟脉灵注射液，是一种由苦碟子（Ixeris sonchifolia Hance，又名苦碟子）为主要成分的中药注射剂，目前已广泛用于治疗冠心病、心绞痛、脑梗死等疾病，但其药理作用机制尚不清楚。本研究旨在探讨KDZI对大脑中动脉闭塞再灌注（MCAO/R）大鼠的影响，并采用基于超高效液相色谱-高分辨质谱（UPLC-HRMS）的非靶向代谢分析方法，研究脑缺血再灌注后代谢特征。在此过程中，在血浆中发现了32种潜在的生物标志物。KDZI显著上调血浆中牛磺鹅去氧胆酸、亮氨酸、L-苯丙氨酸、L-色氨酸、花生四烯酸（ARA）、磷脂酰乙醇胺（PE）、磷脂酰胆碱（PC）的水平，下调L-缬氨酸和5-羟基吲哚-3-乙酸（5-HIAA）的水平。结果表明，KDZI对MCAO/R的作用机制与氨基酸和脂质代谢有关。
Kudiezi injection (KDZI), also known as Diemailing injection, is a traditional Chinese medicine injection of the composite plant Ixeris sonchifolia Hance (also known as Kudiezi), and has been widely used to treat coronary heart disease, angina pectoris, and cerebral infarction, but its pharmacological mechanisms remain unclear. This study is designed to explore the effects of KDZI on middle cerebral artery occlusion and reperfusion (MCAO/R) rats, and to identify metabolic features of cerebral ischemia reperfusion by using a nontargeted metabolic profiling method based on ultra-performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS). In this process, 32 potential biomarkers were found in plasma. KDZI significantly upregulated the levels of taurochenodesoxycholic acid, leucine, l-phenylalanine, l-tryptophan, arachidonic acid (ARA), and phosphatidyl ethanolamines (PE), phosphatidyl cholines (PC) and downregulated the levels of l-valine and 5-hydroxyindole-3-acetic acid (5-HIAA) in plasma. The results indicated that the mechanisms of KDZI on MCAO/R were related to the mechanisms of amino acid and lipid metabolism.
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