Overexpression of NOTCH-regulated ankyrin repeat protein is associated with breast cancer cell proliferation.
Overexpression of NOTCH-regulated ankyrin repeat protein is associated with breast cancer cell proliferation.
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发表时间：
2014-05
影响因子：
2
通讯作者：
T. Imaoka;Tomomi Okutani;K. Daino;D. Iizuka;M. Nishimura;Y. Shimada
中科院分区：
文献类型：
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作者：
T. Imaoka;Tomomi Okutani;K. Daino;D. Iizuka;M. Nishimura;Y. Shimada
背景/目的 NOTCH 调节的锚蛋白重复蛋白 (NRARP) 与发育过程中 NOTCH 和无翼型小鼠乳腺肿瘤病毒整合位点 (WNT) 信号之间的串扰有关。我们的研究旨在阐明其在乳腺癌细胞中的作用。材料和方法 使用公共微阵列数据分析人类和大鼠乳腺癌的基因表达。将短干扰 RNA 引入 MCF7 和 T47D 人乳腺癌细胞中以沉默 NRARP。通过定量聚合酶链反应分析基因表达。结果 NRARP 转录本在各种大鼠乳腺癌模型中普遍过表达。此外，人类乳腺癌的一部分也表达高水平的 NRARP 转录本，这与细胞增殖相关基因的上调呈正相关。沉默 NRARP 可抑制 MCF7 和 T47D 细胞的生长，并降低 MCF7 细胞中细胞周期相关基因的表达。结论 NRARP 可能刺激人类乳腺癌细胞增殖。
BACKGROUND/AIM NOTCH-regulated ankyrin repeat protein (NRARP) has been implicated in crosstalk between NOTCH and wingless-type mouse mammary tumor virus integration site (WNT) signals during development. Our study aimed to clarify its role in breast cancer cells. MATERIALS AND METHODS Public microarray data were used to analyze gene expression in human and rat breast cancer. A short interfering RNA was introduced into MCF7 and T47D human breast cancer cells for NRARP silencing. Gene expression was analyzed by quantitative polymerase chain reaction. RESULTS The NRARP transcript was commonly overexpressed in various rat mammary cancer models. In addition, a subset of human breast cancer also expressed high levels of NRARP transcript, which correlated positively with up-regulation of cell proliferation-related genes. Silencing of NRARP suppressed the growth of MCF7 and T47D cells and lowered the expression of cell cycle-related genes in MCF7 cells. CONCLUSION NRARP may stimulate cell proliferation in human breast cancer.