Angiotensin II Elicits Water Seeking Behavior and the Water Absorption Response in the Toad Bufo bufo
Angiotensin II Elicits Water Seeking Behavior and the Water Absorption Response in the Toad Bufo bufo
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血管紧张素 II 引发蟾蜍的寻水行为和吸水反应
DOI：
10.1006/hbeh.2001.1649
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发表时间：
2001
影响因子：
3.5
通讯作者：
P. Rosenkilde
中科院分区：
文献类型：
--
作者：
A. L. Viborg;P. Rosenkilde
完全水合的蟾蜍，蟾蜍适应模拟的陆地栖息地，获得庇护所和水。为了从避难所到达水中，蟾蜍必须走过Ohaus天平的平底锅，并将体重记录在计算机上。在背淋巴囊注射人血管紧张素II（A II）、Thr（8）-saralasin或Ringer's后，立即将蟾蜍置于庇护所内，并通过视频监控连续记录其行为。注射剂量为溶于0.1 ml林格氏液中的1 - 100 μ g/100 g体重A II和100 μ g/100 g体重萨拉新;对照动物接受相同体积的林格氏液。与对照组相比，注射A-II和saralasin的蟾蜍从注射到开始吸水行为（WR）的潜伏期显著缩短。注射了A-II和saralasin的蟾蜍在水中的时间也比对照组长得多。膀胱贮库时，WR被终止显着大于A-II或saralasin注射蟾蜍比对照组。哺乳动物A Ⅱ拮抗剂Thr（8）-saralasin对B WR行为的刺激作用。蟾蜍暗示两栖动物和哺乳动物之间受体结构和/或受体分布的差异。
Fully hydrated toads, Bufo bufo were acclimated to a simulated terrestrial habitat, with access to shelters and water. To get from the shelters to the water, the toads had to walk across the pan of an Ohaus balance and the body weights were recorded on a computer. Toads were placed inside shelters immediately following injection of human angiotensin II (A II), Thr(8)-saralasin, or Ringer's in the dorsal lymph sac, and their behavior was recorded continuously by video surveillance. The injection doses were 1-100 microg/100 g body weight A II and 100 microg/100 g body weight saralasin dissolved in 0.1 ml Ringer's; control animals received the same volume of Ringer's. The latency from injection to the initiation of water absorption behavior (WR) was significantly shorter in both A-II- and saralasin-injected toads, compared to controls. A-II- and saralasin-injected toads also spent significantly more time in the water than controls. The bladder depots when WR was terminated were significantly larger in A-II- or saralasin-injected toads than in controls. The stimulatory action of Thr(8)-saralasin, an antagonist of A II in mammals, on WR behavior in B. bufo suggests differences in receptor structure and/or receptor distribution between amphibians and mammals.