A simplified precise method for a adjusting serum levels of persistent organohalogen pollutants to total serum lipids
A simplified precise method for a adjusting serum levels of persistent organohalogen pollutants to total serum lipids
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DOI：
10.1016/j.chemosphere.2005.04.107
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发表时间：
2006-01-01
期刊：
影响因子：
8.8
通讯作者：
Hagmar, L
中科院分区：
文献类型：
--
作者：
Rylander, L;Nilsson-Ehle, P;Hagmar, L
为了获得反映持久性有机卤素污染物体内负荷的生物标志物值，血清或血浆中亲脂性持久性有机卤素污染物的浓度通常以脂重为基础，而不是以鲜重为基础。有两种不同的方法来测定血清和血浆中的脂质含量。重量测定法比酶法更昂贵，而且需要更长的经验。大多数医院的临床化学实验室常规进行胆固醇和甘油三酯的酶测定，而磷脂分析不是这些分析的一部分。因此，在本研究中，我们评估了617名个体的血清中甘油三酯、胆固醇和磷脂水平的总和与甘油三酯和胆固醇水平总和之间的关联。甘油三酯和胆固醇的血清浓度总和与总脂质浓度之间存在非常强的线性相关性。高达97.2%的总脂质浓度变化可通过甘油三酯和胆固醇浓度之和进行解释，回归方程如下：总脂质= 0.9 + 1.3 *（胆固醇+甘油三酯）。几乎相同的关系记录在亚组与男性，女性和不同年龄的主题。因此，血清中的总脂质浓度可以通过仅分析基质中的胆固醇和甘油三酯来准确估计。(c)2005爱思唯尔有限公司保留所有权利。
In order to obtain biomarker values that reflect body burden of persistent organohalogen pollutants (POP), concentrations of lipophilic POP in serum or plasma are generally expressed on a lipid weight basis, and not on a fresh weight basis. There are two different approaches to determine the lipid content in serum and plasma. The gravimetric determination is more expensive, and longer experience is needed as compared with the preferable enzymatic determination. Clinical chemistry laboratories at most hospitals perform enzymatic determinations of cholesterol and triglycerides on a routine basis, whereas analysis of phospholipids is not part of these analyses. In the present study, therefore, we evaluated the association between the sum of measured triglyceride, cholesterol, and phospholipid levels in serum among 617 individuals with the sum of only triglyceride and cholesterol levels. There was a very strong linear association between the sums of serum concentrations of triglycerides and cholesterols and the total lipid concentrations. As much as 97.2% of the variation in total lipid concentrations was explained by the sum of the triglyceride and cholesterol concentrations, with the following regression: Total lipid = 0.9 + 1.3 * (Cholesterol + Triglycerides). Almost identical relationships were recorded in subgroups with men, women and subjects of different age. Thus, total lipid concentrations in blood serum can be accurately estimated by analyzing only cholesterol and triglycerides in the matrix. (c) 2005 Elsevier Ltd. All rights reserved.