Observation of DNA molecules using fluorescence microscopy and atomic force microscopy
Observation of DNA molecules using fluorescence microscopy and atomic force microscopy
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DOI：
10.1021/ed085p680
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发表时间：
2008-05-01
影响因子：
3
通讯作者：
Ito, Takashi
中科院分区：
文献类型：
--
作者：
Ito, Takashi
本文介绍了一个本科仪器分析实验室，旨在观察单个双链DNA（dsDNA）分子，使用荧光显微镜和原子力显微镜（AFM）的实验。在几种不同的条件下观察dsDNA分子，以讨论它们的化学和物理性质。特别是，双链DNA分子固定在平面基板上，在其拉伸形式允许学生使用这些显微镜技术测量的双链DNA分子的大小。测量值和文献值之间的比较提供了一种手段，用于教学的显微技术的特点。实验可以在四个小时内完成，适合在有限的实验时间内向本科生介绍这些现代显微镜方法以及纳米技术的基础知识。
This article describes experiments for an undergraduate instrumental analysis laboratory that aim to observe individual double-stranded DNA (dsDNA) molecules using fluorescence microscopy and atomic force microscopy (AFM). dsDNA molecules are observed under several different conditions to discuss their chemical and physical properties. In particular, immobilization of dsDNA molecules on a planar substrate in their stretched form permits students to measure the size of the dsDNA molecules using these microscopic techniques. Comparison between the measured size and literature values provides a means for teaching the characteristics of the microscopic techniques. The experiments can be completed within four hours and are suitable to introduce these modern microscopic methods as well as the basics of nanotechnology to undergraduate students during limited lab hours.