Leukocyte locomotion and chemotaxis. New methods for evaluation, and demonstration of a cell-derived chemotactic factor.
Leukocyte locomotion and chemotaxis. New methods for evaluation, and demonstration of a cell-derived chemotactic factor.
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白细胞运动和趋化性。评估的新方法，并证明了细胞衍生的趋化因子。
DOI：
10.1084/jem.137.2.387
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发表时间：
1973-02-01
影响因子：
15.3
通讯作者：
Hirsch, J G
中科院分区：
文献类型：
--
作者：
Zigmond, S H;Hirsch, J G
通过直接显微镜观察薄载玻片-盖玻片制备物中的单个细胞，以及观察迁移到Millipore过滤器中的细胞群体，评价了多形性白细胞（PMN）的运动和趋化性。直接显微镜方法使用移动的PMNs的极性（宽的、前进的板状伪足和后部的节状收缩）来记录移动的方向。Boyden室Millipore试验通过跟踪细胞前进到过滤器中的前部而不是计数过滤器下表面上的细胞数量而变得更可靠。为了区分对运动速率和真正趋化性的影响，需要对Millipore试验进行特殊修改。在这两个系统中的结果表明，在某些条件下，白细胞，特别是中性粒细胞，释放到介质中的一个因素刺激运动和施加趋化作用的中性粒细胞在附近。这种细胞衍生因子的释放或作用似乎不需要血清因子。
Polymorphonuclear leukocyte (PMN) locomotion and chemotaxis have been evaluated by direct microscopic observation of individual cells in thin slide-cover slip preparations, and also by observations on populations of cells migrating into a Millipore filter. The direct microscopic method used the polarity of the locomoting PMNs (broad, advancing lamellipodium and knoblike constriction at the rear) to record the direction of movement. The Boyden chamber Millipore assay was made more reliable by following the front of cells advancing into the filter, rather than counting the number of cells on the lower filter surface. Special modifications of the Millipore assay were necessary in order to distinguish between influences on rate of locomotion and true chemotaxis. In both systems the results indicate that under certain conditions leukocytes, and in particular PMNs, release into the medium a factor stimulating locomotion and exerting chemotactic action on PMNs in the vicinity. This cell-derived factor appears not to require serum factors for its release or action.