Evaluation of sCMOS cameras for detection and localization of single Cy5 molecules.
Evaluation of sCMOS cameras for detection and localization of single Cy5 molecules.
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DOI：
10.1364/oe.20.007338
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发表时间：
2012-03-26
期刊：
影响因子：
3.8
通讯作者：
Bruchez MP
中科院分区：
文献类型：
--
作者：
Saurabh S;Maji S;Bruchez MP
在电子噪声下检测单分子的能力需要高性能的检测器系统。电子倍增电荷耦合器件 (EMCCD) 相机已成功用于对单分子成像。最近，推出了基于科学互补金属氧化物半导体 (sCMOS) 的相机，与 EMCCD 相机相比，该相机具有极低的读取噪声、更快的读出速率、更小的像素尺寸和更低的价格。在本研究中，我们比较了使用两个 EMCCD 和三个 sCMOS 相机检测单个 Cy5 分子的两种技术。我们的研究结果表明，sCMOS 相机在检测和定位单个 Cy5 分子方面的表现与 EMCCD 相机类似。
The ability to detect single molecules over the electronic noise requires high performance detector systems. Electron Multiplying Charge-Coupled Device (EMCCD) cameras have been employed successfully to image single molecules. Recently, scientific Complementary Metal Oxide Semiconductor (sCMOS) based cameras have been introduced with very low read noise at faster read out rates, smaller pixel sizes and a lower price compared to EMCCD cameras. In this study, we have compared the two technologies using two EMCCD and three sCMOS cameras to detect single Cy5 molecules. Our findings indicate that the sCMOS cameras perform similar to EMCCD cameras for detecting and localizing single Cy5 molecules.