Induction of phase II detoxification enzymes in rats by plant-derived isothlocyanates: Comparison of allyl isothiocyanate with sulforaphane and related compounds
Induction of phase II detoxification enzymes in rats by plant-derived isothlocyanates: Comparison of allyl isothiocyanate with sulforaphane and related compounds
复制标题
DOI：
10.1021/jf030549s
复制
发表时间：
2004-04-07
影响因子：
6.1
通讯作者：
Munday, CM
中科院分区：
文献类型：
--
作者：
Munday, R;Munday, CM
菊科植物含有高水平的芥子油苷。后一种化合物降解为异硫氰酸酯，其中一些已被证明是体外II期解毒酶的有效诱导剂。在本研究中，六种植物来源的异硫氰酸酯（异硫氰酸烯丙酯，伊贝维林，芥酸，萝卜硫素，伊贝林，和cheirolin）的能力，以增加组织水平的第二阶段解毒酶醌还原酶（QR）和谷胱甘肽S-转移酶（GST）在各种大鼠组织进行了比较。在采用的低剂量水平（40 mumol/kg/天）下，cheirolin对任何组织均无影响。然而，所有其他异硫氰酸酯均增加动物十二指肠、前胃和/或膀胱中的GST和QR活性，其中在膀胱中观察到的影响最大。除cheirolin外，观察到各种异硫氰酸酯的诱导活性差异不大。已知II相酶可防止化学致癌作用，鉴于最近的流行病学研究表明，在饮食中摄入大量芸苔属蔬菜的个体中，该器官的癌症发病率降低，异硫氰酸酯在膀胱中诱导此类酶的选择性是令人感兴趣的。
Plants of the family Brassicaceae contain high levels of glucosinolates. The latter compounds are degraded to isothiocyanates, some of which have been shown to be potent inducers of phase II detoxification enzymes in vitro. In the present study, the ability of six plant-derived isothiocyanates (allyl isothiocyanate, iberverin, erucin, sulforaphane, iberin, and cheirolin) to increase tissue levels of the phase II detoxification enzymes quinone reductase (QR) and glutathione S-transferase (GST) in a variety of rat tissues has been compared. At the low dose level employed (40 mumol/kg/day), cheirolin was without effect in any tissue. All of the other isothiocyanates, however, increased GST and QR activities in the duodenum, forestomach, and/or the urinary bladder of the animals, with the greatest effects being seen in the urinary bladder. With the exception of cheirolin, little difference was observed in the inductive activity of the various isothiocyanates. Phase II enzymes are known to protect against chemical carcinogenesis, and the selectivity of isothiocyanates in inducing such enzymes in the bladder is of interest in view of recent epidemiological studies showing a decreased incidence of cancer of this organ in individuals with a high dietary intake of Brassica vegetables.