Proliferation of antibiotic resistance genes in coastal recirculating mariculture system.
Proliferation of antibiotic resistance genes in coastal recirculating mariculture system.
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DOI：
10.1016/j.envpol.2019.02.062
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发表时间：
2019-02
影响因子：
8.9
通讯作者：
Jianhua Wang;Jian Lu;Jun Wu;Yuxuan Zhang;Cui Zhang
中科院分区：
文献类型：
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作者：
Jianhua Wang;Jian Lu;Jun Wu;Yuxuan Zhang;Cui Zhang
抗生素的滥用导致了抗生素抗性基因在水产养殖系统中的传播。虽然循环系统已被认为是一种有前途的方法，防止沿海水域的抗生素和ARG污染，很少有资料提供的ARG在循环海水养殖系统的分布和增殖。本研究首先研究了沿海循环海水养殖系统中ARGs的增殖情况。检测到tet（tetB，tetG，tetX），sul（sul 1，sul 2），qnr（qnrA，qnrB，qnrS）和derm（ermF，ermT）等10种ARGs亚型。养殖场中ARGs的绝对丰度均大于1 × 104 copies/mL。磺胺类药物耐药基因（sul 1和sul 2）的丰度最高，为3.5 × 107 ~ 6.5 × 1010拷贝/mL。抗生素与ARGs无明显相关性。某些细菌与两种或两种以上ARG呈正相关，表明发生了多重耐药。循环水处理系统中的生物流化床是ARGs的主要滋生地，而紫外线杀菌工艺可以降低ARGs的产生。ARGs的最高通量（6.5 × 1021拷贝/d）表明，在循环水养殖系统中，粪便和残留饵料的排放是ARGs进入环境的主要途径。
The abuse of antibiotics has caused the propagation of antibiotic resistance genes (ARGs) in aquaculture systems. Although the recirculating systems have been considered as a promising approach for preventing the coastal water pollution of antibiotics and ARG, rare information is available on the distribution and proliferation of ARGs in the recirculating mariculture system. This study firstly investigated the proliferation of ARGs in coastal recirculating mariculture systems. Ten subtypes of ARGs includingtet(tetB,tetG,tetX),sul(sul1,sul2),qnr(qnrA,qnrB,qnrS), anderm(ermF,ermT) were detected. The absolute abundances of the ARGs detected in the mariculture farm were more than 1 × 104copies/mL. The sulfonamide resistance genes (sul1 andsul2) were the most abundant ARGs with the abundance of 3.5 × 107–6.5 × 1010copies/mL. No obvious correlation existed between the antibiotics and ARGs. Some bacteria were positively correlated with two or more ARGs to indicate the occurrence of multidrug resistance. The fluidized-bed biofilter for wastewater treatment in the recirculating system was the main breeding ground for ARGs while the UV sterilization process could reduce the ARGs. The highest flux of ARGs (6.5 × 1021copies/d) indicated that the discharge of feces and residual baits was the main gateway for ARGs in the recirculating mariculture system to enter the environments.